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Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

  • Aline Do Minh
  • Michelle Yen Tran
  • Amine A. KamenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2086)

Abstract

The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.

Key words

Lentiviral vectors Serum-free medium Suspension cells Transient transfection Packaging cells Producer cells 

Notes

Acknowledgments

The authors would like to acknowledge Rénald Gilbert, Sven Ansorge, and their respective teams at NRC for providing HEK293 packaging and producer cell lines to support the research program with lentiviral vectors as well as funding from the Canada Research Chair (CRC-2403940) and Canadian Foundation for Innovation (CFI-32904).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Aline Do Minh
    • 1
  • Michelle Yen Tran
    • 1
  • Amine A. Kamen
    • 1
    Email author
  1. 1.Department of BioengineeringMcGill UniversityMontrealCanada

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