Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses
This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing adapters for high-throughput sequencing. This protocol will be useful for all double-stranded RNA viruses and for all of the high-throughput sequencing platforms.
Key wordsEfficient sequencing protocols dsRNA viruses Enzyme treatment Precipitation by LiCl FL amplification
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