The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines
Differential mobility spectrometry (DMS) is capable of separating molecules based on their size and shape. When coupled to mass spectrometry (MS), DMS reduces chemical background and enhances signal-to-noise (S/N) ratio. Flow injection analysis (FIA) is a technique used to introduce samples into the source of the DMS-MS platform. Here we describe the application of FIA-DMS-MS/MS for the analysis of urinary acylcarnitine species. More than 20 acylcarnitine species can be detected and quantified during a single FIA-DMS-MS/MS acquisition.
Key wordsDifferential mobility spectrometry Mass spectrometry Acylcarnitine Solid-phase extraction
This work was funded by the Internal Medicine Research Unit (IMRU), within the Pfizer Global Research and Development Business Unit, at Pfizer.
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