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In Situ Determination of Genotoxic Effects in Fish Erythrocytes Using Comet and Micronucleus Assays

  • Paula Suares Rocha
  • Björn Deutschmann
  • Henner Hollert
Protocol
  • 24 Downloads
Part of the Methods in Pharmacology and Toxicology book series

Abstract

Genotoxic pollutants are a major concern nowadays, since they can interact with the genetic material of exposed organisms, which may initiate a cascade of damages that may lead to endangerment from molecular to the community levels. The comet and the micronucleus assays performed with fish erythrocytes can be used to identify extrachromosomal DNA adducts in situ resulting from strand breaks, chromosomal breaks, or aneugenic effects. These two assays, performed as both in vitro and in situ, have been successfully applied to detect genotoxic potentials of environmental samples in several monitoring studies. In situ bioassays reflect the real exposure situation of an environment; thus, they may be useful to confirm or falsify the potential toxicity revealed by in vitro bioassays. Hence, this chapter presents a general guideline to perform in situ comet and micronucleus assays, using fish erythrocytes, as well as some important observations that can be used to minimize possible technical errors.

Keywords

In situ investigation Genotoxicity DNA damage Complex mixtures 

Notes

Acknowledgments

Björn Deutschmann was financed by SOLUTIONS project (European Union’s Seventh Framework Program for research, technological development, and demonstration), under Grant Agreement No. 603437.

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Copyright information

© Springer Science+Business Media, LLC 2019

Authors and Affiliations

  • Paula Suares Rocha
    • 1
    • 2
  • Björn Deutschmann
    • 1
  • Henner Hollert
    • 1
    • 2
  1. 1.RWTH Aachen UniversityAachenGermany
  2. 2.Faculty of TechnologyUNICAMPLimeiraBrazil

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