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Fluorescent Nanoclusters for Imaging of Cells/Stem Cells

  • Fatemeh Molaabasi
  • Behnam Hajipour-Verdom
  • Mozhgan Alipour
  • Seyed Morteza NaghibEmail author
Protocol
  • 84 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)

Abstract

Imaging of cancer cells and cancer stem cells (CSCs) plays an important role in studying cell biology and tracking cancer development and metastasis. There is a wide interest in targeting cancer cells using fluorescent nanoclusters (NCs) capped in protein due to excellent cell viability and photostability, one-step synthesis route, large Stokes shift, good aqueous stability, and easy functionalization capability with long lifetime. Since CD44 is a CSC marker as well as a transmembrane receptor for hyaluronic acid (HA) and many other extracellular matrix (ECM) components, in this protocol, a biocompatible platform was synthesized by conjugation of HA onto luminescent platinum nanoclusters (Pt NCs) in human hemoglobin (Hb) (Hb/Pt NCs). This bioplatform could be used for specific imaging via an efficient targeting of CD44-overexpressing cancer cells and cancer stem cells.

Keywords

CD44 marker Cell/stem cell Human hemoglobin Hyaluronic acid Luminescent platinum nanoclusters 

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Fatemeh Molaabasi
    • 1
  • Behnam Hajipour-Verdom
    • 2
  • Mozhgan Alipour
    • 2
  • Seyed Morteza Naghib
    • 3
    Email author
  1. 1.Department of Biomaterials and Tissue Engineering, Breast Cancer Research CenterMotamed Cancer Institute, ACECRTehranIran
  2. 2.Department of Biophysics, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  3. 3.Nanotechnology Department, School of Advanced TechnologiesIran University of Science and Technology (IUST)TehranIran

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