Fluorescent Nanoclusters for Imaging of Cells/Stem Cells
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Imaging of cancer cells and cancer stem cells (CSCs) plays an important role in studying cell biology and tracking cancer development and metastasis. There is a wide interest in targeting cancer cells using fluorescent nanoclusters (NCs) capped in protein due to excellent cell viability and photostability, one-step synthesis route, large Stokes shift, good aqueous stability, and easy functionalization capability with long lifetime. Since CD44 is a CSC marker as well as a transmembrane receptor for hyaluronic acid (HA) and many other extracellular matrix (ECM) components, in this protocol, a biocompatible platform was synthesized by conjugation of HA onto luminescent platinum nanoclusters (Pt NCs) in human hemoglobin (Hb) (Hb/Pt NCs). This bioplatform could be used for specific imaging via an efficient targeting of CD44-overexpressing cancer cells and cancer stem cells.
KeywordsCD44 marker Cell/stem cell Human hemoglobin Hyaluronic acid Luminescent platinum nanoclusters
- 6.Jiang K, Chi T, Li T, Zheng G, Fan L, Liu Y, Chen X, Chen S, Jia L, Shao J (2017) A smart pH-responsive nano-carrier as a drug delivery system for the targeted delivery of ursolic acid: suppresses cancer growth and metastasis by modulating P53/MMP-9/PTEN/CD44 mediated multiple signaling pathways. Nanoscale 9(27):9428–9439CrossRefGoogle Scholar
- 10.Shamsipur M, Molaabasi F, Sarparast M, Roshani E, Vaezi Z, Alipour M, Molaei K, Naderi-Manesh H, Hosseinkhani S (2018) Photoluminescence mechanisms of dual-emission fluorescent silver nanoclusters fabricated by human hemoglobin template: from oxidation-and aggregation-induced emission enhancement to targeted drug delivery and cell imaging. ACS Sustain Chem Eng 6(8):11123–11137CrossRefGoogle Scholar
- 12.Molaabasi F, Sarparast M, Shamsipur M, Irannejad L, Moosavi-Movahedi AA, Ravandi A, Verdom BH, Ghazfar R (2018) Shape-controlled synthesis of luminescent hemoglobin capped hollow porous platinum nanoclusters and their application to catalytic oxygen reduction and cancer imaging. Sci Rep 8(1):14507CrossRefGoogle Scholar