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Dermal-Epidermal Separation by Heat

  • Liyan Jian
  • Yu Cao
  • Ying Zou
Protocol
Part of the Methods in Molecular Biology book series

Abstract

The skin contains three primary layers: epidermis, dermis, and hypodermis. Separation of epidermal components from the dermis (dermal-epidermal separation) is an important basic investigation technique for pharmacology, toxicology, and biology. There are different systems of epidermal separation, including typical methods of chemical, enzyme, heat, etc. Each approach has advantages versus disadvantages, and thus the appropriate method should be chosen for a given research question. Here we described the method of heat separation.

Keywords

Epidermis Dermis Separation Heat 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC) grant No. 81602757, the Science and Technology Commission of Shanghai Municipality grant No. 16411961800, and the Science Foundation of Shanghai Hospital Development Center grant No. 16CR3096B.

References

  1. 1.
    Zou Y, Maibach HI (2018) Dermal-epidermal separation methods: research implications. Arch Dermatol Res 310(1):1–9Google Scholar
  2. 2.
    Baumberger JP, Suntzeff V, Cowdry EV (1942) Methods for separation of epidermis from dermis and some physiologic and chemical properties of isolated epidermis. J Natl Cancer Inst 2:413–416Google Scholar
  3. 3.
    Ohata Y, Hashimoto T, Nishikawa T (1995) Comparative study of autoantigens for various bullous skin diseases by immunoblotting using different dermo-epidermal separation techniques. Clin Exp Dermatol 20:454–458Google Scholar
  4. 4.
    Wadskov S, Sondergaard J (1978) Determination of cyclic AMP in heat-separated human epidermal tissue. Acta Derm Venereol 58:191–195Google Scholar
  5. 5.
    Tsambaos D, Mahrle G (1979) The phospholipid pattern in the involved and the uninvolved psoriatic epidermis. Arch Dermatol Res 266:177–180Google Scholar
  6. 6.
    Kassis V, Sondergaard J (1982) Heat-separation of normal human skin for epidermal and dermal prostaglandin analysis. Arch Dermatol Res 273:301–306Google Scholar
  7. 7.
    Briggaman RA, Wheeler CE Jr (1975) The epidermal-dermal junction. J Invest Dermatol 65:71–84Google Scholar
  8. 8.
    Fort JJ, Mitra AK (1994) Effects of epidermal/dermal separation methods and ester chain configuration on the bioconversion of a homologous series of methotrexate dialkyl esters in dermal and epidermal homogenates of hairless mouse skin. Int J Pharm 102:241–247Google Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Liyan Jian
    • 1
  • Yu Cao
    • 1
  • Ying Zou
    • 2
  1. 1.Institute of Precision Medicine, The Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Skin & Cosmetic Research DepartmentShanghai Dermatology HospitalShanghaiChina

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