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Dermal-Epidermal Separation by Heat

  • Liyan Jian
  • Yu Cao
  • Ying Zou
Part of the Methods in Molecular Biology book series


The skin contains three primary layers: epidermis, dermis, and hypodermis. Separation of epidermal components from the dermis (dermal-epidermal separation) is an important basic investigation technique for pharmacology, toxicology, and biology. There are different systems of epidermal separation, including typical methods of chemical, enzyme, heat, etc. Each approach has advantages versus disadvantages, and thus the appropriate method should be chosen for a given research question. Here we described the method of heat separation.


Epidermis Dermis Separation Heat 



This work was supported by the National Natural Science Foundation of China (NSFC) grant No. 81602757, the Science and Technology Commission of Shanghai Municipality grant No. 16411961800, and the Science Foundation of Shanghai Hospital Development Center grant No. 16CR3096B.


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Liyan Jian
    • 1
  • Yu Cao
    • 1
  • Ying Zou
    • 2
  1. 1.Institute of Precision Medicine, The Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Skin & Cosmetic Research DepartmentShanghai Dermatology HospitalShanghaiChina

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