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Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease

  • Nareshwaran Gnanasegaran
  • Deepak B. Thimiri Govinda Raj
  • Sivakumar Arumugam
Part of the Methods in Molecular Biology book series


Several research groups have utilized dental pulp stem cells for numerous studies as treatment modality for Parkinson’s disease (PD). However, the roles of dental pulp stem cells in governing the Parkinson’s disease inflammatory microenvironment remain to be evaluated. In this article, we elaborate the method where we can investigate the effects of dental pulp stem cells on neurons and microglia in an in vitro inflammatory microenvironment.


Dental pulp stem cell SHED Parkinson’s disease (PD) Deciduous tooth Regenerative medicine 



This work was supported by University of Malaya, Kuala Lumpur, Malaysia. Authors thank scientific input and relevant discussions from Envirotransgene® Global, Chennai, India.


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Nareshwaran Gnanasegaran
    • 1
    • 2
  • Deepak B. Thimiri Govinda Raj
    • 3
  • Sivakumar Arumugam
    • 4
  1. 1.Agency for Science, Technology and Research (A∗STAR)SingaporeSingapore
  2. 2.Faculty of DentistryUniversity of MalayaKuala LumpurMalaysia
  3. 3.Envirotransgene Biosolutions GlobalChennaiIndia
  4. 4.School of Bio Sciences and Technology (SBST), VIT UniversityVelloreIndia

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