Scratch Wound Healing Assay

Part of the Methods in Molecular Biology book series (MIMB, volume 2109)


Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.


Cell migration Data analysis Healing assay Scratch wound assay Wound repair 


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.DiSIT-Dipartimento di Scienze e Innovazione TecnologicaUniversity of Piemonte OrientaleVercelliItaly

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