Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles

  • Taha Umair Wani
  • Hasham S. Sofi
  • Nisar Ahmad Khan
  • Faheem A. SheikhEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)


Therapy based on stem cells utilizes these cells in neurodegeneration, brain/spinal cord injury, and much recently in repairing of severe heart diseases. Owning to their stemness, these cells are the potential source of progenitors that can offer a therapeutic remedy to a variety of diseases and/or disorders. The ability of these cells to regenerate and differentiate into specified phenotypes has great utility in tissue regeneration applications. This chapter provides a detailed account for isolation of neural stem cells from the mice embryo. Furthermore, the fabrication of chitosan–tripolyphosphate/hyaluronic acid-based nanoparticles and evaluating their efficiency in inducing transfection in the isolated neural stem cells as an approach for the treatment of neurodegenerative disorders.


Nanoparticles Self-renewal Therapeutic agents 



The findings presented here are financially supported by Science and Engineering Research Board (SERB) under grant number ECR/2016/001429.


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Taha Umair Wani
    • 1
  • Hasham S. Sofi
    • 2
  • Nisar Ahmad Khan
    • 1
  • Faheem A. Sheikh
    • 2
    Email author
  1. 1.Department of Pharmaceutical SciencesUniversity of KashmirSrinagarIndia
  2. 2.Department of NanotechnologyUniversity of KashmirSrinagarIndia

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