Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles
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Therapy based on stem cells utilizes these cells in neurodegeneration, brain/spinal cord injury, and much recently in repairing of severe heart diseases. Owning to their stemness, these cells are the potential source of progenitors that can offer a therapeutic remedy to a variety of diseases and/or disorders. The ability of these cells to regenerate and differentiate into specified phenotypes has great utility in tissue regeneration applications. This chapter provides a detailed account for isolation of neural stem cells from the mice embryo. Furthermore, the fabrication of chitosan–tripolyphosphate/hyaluronic acid-based nanoparticles and evaluating their efficiency in inducing transfection in the isolated neural stem cells as an approach for the treatment of neurodegenerative disorders.
KeywordsNanoparticles Self-renewal Therapeutic agents
The findings presented here are financially supported by Science and Engineering Research Board (SERB) under grant number ECR/2016/001429.
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