Advertisement

Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells Through Polymeric Nanoparticles

  • Taha Umair Wani
  • Hasham S. Sofi
  • Nisar Ahmad Khan
  • Faheem A. SheikhEmail author
Protocol
  • 298 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)

Abstract

Therapy based on stem cells utilizes these cells in neurodegeneration, brain/spinal cord injury, and much recently in repairing of severe heart diseases. Owning to their stemness, these cells are the potential source of progenitors that can offer a therapeutic remedy to a variety of diseases and/or disorders. The ability of these cells to regenerate and differentiate into specified phenotypes has great utility in tissue regeneration applications. This chapter provides a detailed account for isolation of neural stem cells from the mice embryo. Furthermore, the fabrication of chitosan–tripolyphosphate/hyaluronic acid-based nanoparticles and evaluating their efficiency in inducing transfection in the isolated neural stem cells as an approach for the treatment of neurodegenerative disorders.

Keywords

Nanoparticles Self-renewal Therapeutic agents 

Notes

Acknowledgments

The findings presented here are financially supported by Science and Engineering Research Board (SERB) under grant number ECR/2016/001429.

References

  1. 1.
    Mahla RS (2016) Stem cells applications in regenerative medicine and disease therapeutics. Int J Cell Biol 2016:6940283CrossRefGoogle Scholar
  2. 2.
    Trounson A, DeWitt ND (2016) Pluripotent stem cells progressing to the clinic. Nat Rev Mol Cell Biol 17(3):194CrossRefGoogle Scholar
  3. 3.
    Tao H, Han Z, Han ZC, Li Z (2016) Proangiogenic features of mesenchymal stem cells and their therapeutic applications. Stem Cells Int 2016:1314709CrossRefGoogle Scholar
  4. 4.
    Hyslop LA, Blakeley P, Craven L, Richardson J, Fogarty NM, Fragouli E, Lamb M, Wamaitha SE, Prathalingam N, Zhang Q (2016) Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature 534(7607):383CrossRefGoogle Scholar
  5. 5.
    Wankhade UD, Shen M, Kolhe R, Fulzele S (2016) Advances in adipose-derived stem cells isolation, characterization, and application in regenerative tissue engineering. Stem Cells Int 2016:3206807CrossRefGoogle Scholar
  6. 6.
    Rana D, Zreiqat H, Benkirane-Jessel N, Ramakrishna S, Ramalingam M (2017) Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med 11(4):942–965CrossRefGoogle Scholar
  7. 7.
    Dai R, Wang Z, Samanipour R, Koo K-I, Kim K (2016) Adipose-derived stem cells for tissue engineering and regenerative medicine applications. Stem Cells Int 2016:6737345PubMedPubMedCentralGoogle Scholar
  8. 8.
    Mohammadi M, Jaafari M, Mirzaei H, Mirzaei H (2016) Mesenchymal stem cell: a new horizon in cancer gene therapy. Cancer Gene Ther 23(9):285CrossRefGoogle Scholar
  9. 9.
    Kim SU, De Vellis J (2009) Stem cell-based cell therapy in neurological diseases: a review. J Neurosci Res 87(10):2183–2200CrossRefGoogle Scholar
  10. 10.
    Myers TJ, Granero-Molto F, Longobardi L, Li T, Yan Y, Spagnoli A (2010) Mesenchymal stem cells at the intersection of cell and gene therapy. Expert Opin Biol Ther 10(12):1663–1679CrossRefGoogle Scholar
  11. 11.
    Lindvall O, Barker RA, Brüstle O, Isacson O, Svendsen CN (2012) Clinical translation of stem cells in neurodegenerative disorders. Cell Stem Cell 10(2):151–155CrossRefGoogle Scholar
  12. 12.
    Doss MX, Koehler CI, Gissel C, Hescheler J, Sachinidis A (2004) Embryonic stem cells: a promising tool for cell replacement therapy. J Cell Mol Med 8(4):465–473CrossRefGoogle Scholar
  13. 13.
    Watt FM, Huck WT (2013) Role of the extracellular matrix in regulating stem cell fate. Nat Rev Mol Cell Biol 14(8):467CrossRefGoogle Scholar
  14. 14.
    L Santos J, Pandita D, Rodrigues J, P Pego A, L Granja P, Tomás H (2011) Non-viral gene delivery to mesenchymal stem cells: methods, strategies and application in bone tissue engineering and regeneration. Curr Gene Ther 11(1):46–57CrossRefGoogle Scholar
  15. 15.
    McMahon J, Conroy S, Lyons M, Greiser U, O'shea C, Strappe P, Howard L, Murphy M, Barry F, O'brien T (2006) Gene transfer into rat mesenchymal stem cells: a comparative study of viral and nonviral vectors. Stem Cells Dev 15(1):87–96CrossRefGoogle Scholar
  16. 16.
    Zhu K, Wu M, Lai H, Guo C, Li J, Wang Y, Chen Y, Wang C, Shi J (2016) Nanoparticle-enhanced generation of gene-transfected mesenchymal stem cells for in vivo cardiac repair. Biomaterials 74:188–199CrossRefGoogle Scholar
  17. 17.
    Park JS, Yang HN, Yi SW, Kim J-H, Park K-H (2016) Neoangiogenesis of human mesenchymal stem cells transfected with peptide-loaded and gene-coated PLGA nanoparticles. Biomaterials 76:226–237CrossRefGoogle Scholar
  18. 18.
    Tenkumo T, Vanegas Sáenz JR, Takada Y, Takahashi M, Rotan O, Sokolova V, Epple M, Sasaki K (2016) Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite–collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles. Genes Cells 21(7):682–695CrossRefGoogle Scholar
  19. 19.
    Das J, Choi Y-J, Yasuda H, Han JW, Park C, Song H, Bae H, Kim J-H (2016) Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation. Sci Rep 6:33784CrossRefGoogle Scholar
  20. 20.
    Xu S, De Becker A, Van Camp B, Vanderkerken K, Van Riet I (2010) An improved harvest and in vitro expansion protocol for murine bone marrow-derived mesenchymal stem cells. Biomed Res Int 2010:105940Google Scholar
  21. 21.
    Barry FP, Murphy JM (2004) Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 36(4):568–584CrossRefGoogle Scholar
  22. 22.
    Lindvall O, Kokaia Z (2006) Stem cells for the treatment of neurological disorders. Nature 441(7097):1094CrossRefGoogle Scholar
  23. 23.
    Lindvall O, Kokaia Z, Martinez-Serrano A (2004) Stem cell therapy for human neurodegenerative disorders—how to make it work. Nat Med 10(7s):S42CrossRefGoogle Scholar
  24. 24.
    Lindvall O, Kokaia Z (2010) Stem cells in human neurodegenerative disorders—time for clinical translation? J Clin Invest 120(1):29–40CrossRefGoogle Scholar
  25. 25.
    Szymańska E, Winnicka K (2015) Stability of chitosan—a challenge for pharmaceutical and biomedical applications. Mar Drugs 13(4):1819–1846CrossRefGoogle Scholar
  26. 26.
    Dotto GL, Campana-Filho SP, de Almeida Pinto LA (2017) Frontiers in biomaterials: chitosan based materials and its applications, vol 3. Bentham Science Publishers, SharjahCrossRefGoogle Scholar
  27. 27.
    Chang W, Little J (1991) Delayed reproductive death in X-irradiated Chinese hamster ovary cells. Int J Radiat Biol 60(3):483–496CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Taha Umair Wani
    • 1
  • Hasham S. Sofi
    • 2
  • Nisar Ahmad Khan
    • 1
  • Faheem A. Sheikh
    • 2
    Email author
  1. 1.Department of Pharmaceutical SciencesUniversity of KashmirSrinagarIndia
  2. 2.Department of NanotechnologyUniversity of KashmirSrinagarIndia

Personalised recommendations