Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules
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Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.
KeywordsAuNPs SPMNPs Bio-conjugation Phospholipids
This work was supported by Envirotransgene® Global, Chennai, India, and Deepak Thimiri Consulting Group (DTCG®), Chennai, India. Authors thank the infrastructural support from Bannari Amman Institute of Technology, India.
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