Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

  • Deepak B. Thimiri Govinda RajEmail author
  • Niamat Ali Khan
  • Srisaran Venkatachalam
  • Sivakumar Arumugam
  • Selvamani Palaniswamy
Part of the Methods in Molecular Biology book series (MIMB, volume 2125)


Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.


AuNPs SPMNPs Bio-conjugation Phospholipids 



This work was supported by Envirotransgene® Global, Chennai, India, and Deepak Thimiri Consulting Group (DTCG®), Chennai, India. Authors thank the infrastructural support from Bannari Amman Institute of Technology, India.


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Deepak B. Thimiri Govinda Raj
    • 1
    • 2
    Email author
  • Niamat Ali Khan
    • 1
    • 2
  • Srisaran Venkatachalam
    • 3
  • Sivakumar Arumugam
    • 4
  • Selvamani Palaniswamy
    • 5
  1. 1.Envirotransgene® Bio-solutions GlobalChennaiIndia
  2. 2.DTCG®ChennaiIndia
  3. 3.Bannari Amman Institute of TechnologySathyamangalamIndia
  4. 4.School of Bio Sciences and Technology (SBST), VIT UniversityVelloreIndia
  5. 5.Department of Pharmaceutical TechnologyAnna UniversityTiruchirappalliIndia

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