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Methods for Analysis of Keratinocyte Migration

  • Jie Liu
  • Jiaping Zhang
Part of the Methods in Molecular Biology book series


Reepithelialization of wounds involves several keratinocyte functions: proliferation, migration, and differentiation. The key step is keratinocyte migration, an essential aspect for understanding chronic non-healing wounds. Scratch wounding assay and cell motility assay are used to evaluate keratinocyte migration in vitro. The ability of keratinocyte migration is measured by the dynamic change of the wound closure area over time under a living-cell workstation. NIH ImageJ software is used to quantify the motility ability of single cell. The following protocols offer a general guideline for keratinocyte migration analysis.


Cell motility Keratinocyte migration Primary keratinocyte Scratch wounding Wound healing 


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Jie Liu
    • 1
  • Jiaping Zhang
    • 2
  1. 1.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest HospitalArmy Military Medical UniversityChongqingChina
  2. 2.State Key Laboratory of Trauma, Burns and Combined Injury, Plastic Surgery Department, Southwest HospitalArmy Military Medical UniversityChongqingChina

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