Advertisement

pp 1-6 | Cite as

Methods for Analysis of Keratinocyte Migration

  • Jie Liu
  • Jiaping Zhang
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Reepithelialization of wounds involves several keratinocyte functions: proliferation, migration, and differentiation. The key step is keratinocyte migration, an essential aspect for understanding chronic non-healing wounds. Scratch wounding assay and cell motility assay are used to evaluate keratinocyte migration in vitro. The ability of keratinocyte migration is measured by the dynamic change of the wound closure area over time under a living-cell workstation. NIH ImageJ software is used to quantify the motility ability of single cell. The following protocols offer a general guideline for keratinocyte migration analysis.

Keywords

Cell motility Keratinocyte migration Primary keratinocyte Scratch wounding Wound healing 

References

  1. 1.
    Park S, Gonzalez DG, Guirao B, Boucher JD, Cockburn K, Marsh ED et al (2017) Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice. Nat Cell Biol 19:155–163Google Scholar
  2. 2.
    Shaw TJ, Martin P (2009) Wound repair at a glance. J Cell Sci 122:3209–3213Google Scholar
  3. 3.
    Jiang XP, Zhang DX, Teng M, Zhang Q, Zhang JP, Huang YS (2013) Downregulation of CD9 in keratinocyte contributes to cell migration via upregulation of matrix metalloproteinase-9. PLoS One 8:e77806Google Scholar
  4. 4.
    Jiang X, Guo X, Xu X, Teng M, Huang C, Zhang D et al (2014) Hypoxia regulates CD9-mediated keratinocyte migration via the P38/MAPK pathway. Sci Rep 4:6304Google Scholar
  5. 5.
    Guo X, Jiang X, Ren X, Sun H, Zhang D, Zhang Q et al (2015) The galvanotactic migration of keratinocytes is enhanced by hypoxic preconditioning. Sci Rep 5:10289Google Scholar
  6. 6.
    Liu J, Zhu G, Jia N, Wang W, Wang Y, Yin M et al (2019) CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17. Int J Biol Sci 15:493–506Google Scholar
  7. 7.
    Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2:329–333Google Scholar
  8. 8.
    Liu J, Guo X, Ren X, Tian H, Liang Y, Luo Z et al (2018) A novel FPCL model producing directional contraction through induction of fibroblast alignment by biphasic pulse direct current electric field. Exp Cell Res 371:426–434Google Scholar
  9. 9.
    Zhang J, Calafiore M, Zeng Q, Zhang X, Huang Y, Li RA et al (2011) Electrically guiding migration of human induced pluripotent stem cells. Stem Cell Rev 7:987–996Google Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Jie Liu
    • 1
  • Jiaping Zhang
    • 2
  1. 1.Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest HospitalArmy Military Medical UniversityChongqingChina
  2. 2.State Key Laboratory of Trauma, Burns and Combined Injury, Plastic Surgery Department, Southwest HospitalArmy Military Medical UniversityChongqingChina

Personalised recommendations