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pp 1-9 | Cite as

Cell-Extracellular Matrix Adhesion Assay

  • Mehmet VarolEmail author
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Adhesion is defined as a fundamental ability of the adherent cells isolated from the multicellular organisms to attach to an extracellular matrix or another cell, and it takes a key role in a wide variety of the important molecular mechanisms such as cell communication, regulation, differentiation, migration, wound healing, immune response, inflammation, embryonic development, and maintenance of tissues. Adhesion assays therefore provide information about not only the interactions between cell-cell and cell-extracellular matrix but also the other cellular events. In this chapter, it was desired to describe an easily applicable cell-extracellular matrix adhesion assay by explaining the purposes of each experimental step because it seems, to the best of our knowledge, that there is no a complete protocol that explains well the purposes and the related molecular mechanisms of the experimental steps though there are many well-written protocols for adhesion assays.

Keywords

Actin Adhesion Filamin Focal adhesion complex Integrin LDV motif RGD motif Talin Tensin Vinculin 

References

  1. 1.
    Khalili A, Ahmad M (2015) A review of cell adhesion studies for biomedical and biological applications. Int J Mol Sci 16:18149–18184Google Scholar
  2. 2.
    Humphries MJ (2001) Cell adhesion assays. Mol Biotechnol 18:57–61Google Scholar
  3. 3.
    Kucik DF, Wu C (2005) Cell-adhesion assays, cell migration. Springer, Berlin, pp 43–54Google Scholar
  4. 4.
    Scott WN (2004) Cell adhesion assays, cancer cell culture. Springer, Berlin, pp 203–217Google Scholar
  5. 5.
    Wozniak MA, Modzelewska K, Kwong L, Keely PJ (2004) Focal adhesion regulation of cell behavior. Biochim Biophys Acta 1692:103–119Google Scholar
  6. 6.
    Freshney RI (2015) Culture of animal cells: a manual of basic technique and specialized applications. Wiley, New YorkGoogle Scholar
  7. 7.
    Lavia P, Jansen-Dürr P (1999) E2F target genes and cell-cycle checkpoint control. Bioessays 21:221–230Google Scholar
  8. 8.
    Ryan JA (2008) Evolution of cell culture surfaces. BioFiles 3:21Google Scholar
  9. 9.
    Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E (1985) Vitronectin—a major cell attachment-promoting protein in fetal bovine serum. Exp Cell Res 160:245–258Google Scholar
  10. 10.
    Selvaraj A, Prywes R (2004) Expression profiling of serum inducible genes identifies a subset of SRF target genes that are MKL dependent. BMC Mol Biol 5:13Google Scholar
  11. 11.
    Weinberg RA (2014) Cytoplasmic signaling circuitry programs many of the traits of cancer. In: Weinberg RA (ed) The biology of cancer. Garland Science, Taylor & Francis Group, New York, pp 175–229Google Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics, Faculty of ScienceMugla Sitki Kocman UniversityMuglaTurkey

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