Cell-Extracellular Matrix Adhesion Assay

  • Mehmet VarolEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2109)


Adhesion is defined as a fundamental ability of the adherent cells isolated from the multicellular organisms to attach to an extracellular matrix or another cell, and it takes a key role in a wide variety of the important molecular mechanisms such as cell communication, regulation, differentiation, migration, wound healing, immune response, inflammation, embryonic development, and maintenance of tissues. Adhesion assays therefore provide information about not only the interactions between cell-cell and cell-extracellular matrix but also the other cellular events. In this chapter, it was desired to describe an easily applicable cell-extracellular matrix adhesion assay by explaining the purposes of each experimental step because it seems, to the best of our knowledge, that there is no a complete protocol that explains well the purposes and the related molecular mechanisms of the experimental steps though there are many well-written protocols for adhesion assays.


Actin Adhesion Filamin Focal adhesion complex Integrin LDV motif RGD motif Talin Tensin Vinculin 


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics, Faculty of ScienceMugla Sitki Kocman UniversityMuglaTurkey

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