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Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential

  • Carmen Segrelles
  • Karla Santos-de-Frutos
  • Jesús M. Paramio
  • Corina LorzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2109)

Abstract

Epidermal stem cells are responsible for normal tissue homeostasis and contribute to tissue regeneration during injury. Several assays measuring stem cell frequency and function can be used to assess epidermal stem cell potential. However, the ultimate assay that accounts for stemness is the capacity to sustain in vivo long-term tissue regeneration and maintenance. We can use this type of analysis to interrogate whether a specific genetic alteration (e.g., activation or inactivation of any gene thought to be involved in stem cell quiescence or proliferation) confers increased or decreased stem cell potential.

Keywords

Epidermal stem cells Keratinocyte transplantation Skin regeneration Silicone chambers 

Notes

Acknowledgments

Work in our laboratory is supported by FEDER cofounded ISCIII grants [CB16/12/00228 and PI18/00263] and Foundation of Fanconi Anemia grant [2018/127].

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Carmen Segrelles
    • 1
    • 2
    • 3
  • Karla Santos-de-Frutos
    • 1
    • 2
    • 3
  • Jesús M. Paramio
    • 1
    • 2
    • 3
  • Corina Lorz
    • 1
    • 2
    • 3
    Email author
  1. 1.Molecular Oncology Unit, CIEMAT (ed 70A)MadridSpain
  2. 2.Molecular OncologyUniversity Hospital 12 de Octubre, Research Institute 12 de Octubre i+12MadridSpain
  3. 3.Centro de Investigación Biomédica en Red de Cáncer (CIBERONC)MadridSpain

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