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Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application

  • Parisa Goodarzi
  • Hamid Reza Aghayan
  • Moloud Payab
  • Bagher Larijani
  • Sepideh Alavi-Moghadam
  • Masoumeh Sarvari
  • Hossein Adibi
  • Fatemeh Khatami
  • Najmeh Foroughi Heravani
  • Mahdieh Hadavandkhani
  • Babak ArjmandEmail author
Protocol
  • 169 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2109)

Abstract

Cell therapy is one of the most hopeful technologies of regenerative medicine approaches. Among various cells, human skin fibroblasts have been progressively used for wound healing as cell-based therapy purposes. By increasing the age, the number of skin fibroblasts’ abilities including cell migration, growth, collagen production, etc. decreases. Hence, use of the fetal source is more beneficent. In this respect, this chapter covers the manufacturing of human fetal skin-derived fibroblasts for clinical application.

Keywords

Fibroblasts Fetal tissue Cell isolation Culture Therapeutic use 

Abbreviations

bFGF

Basic fibroblast growth factor

CBMPs

Cell-based medicinal products

CD

Cluster of differentiation

cGMP

Current good manufacturing practice

CPMP

Committee for Proprietary Medicinal Product

CVMP

Committee for Medicinal Products for Veterinary Use

DMEM-LG

Dulbecco’s Modified Eagle’s Medium-low glucose

DMSO

Dimethyl sulfoxide

EMA

European Medicines Agency

FBS

Fetal bovine serum

HBV

Hepatitis B virus

HCV

Hepatitis C virus

HEPA

High efficiency particulate air

HIV

Human immunodeficiency virus

kGy

Kilogray

LMP

Last menstrual period

PBS

Phosphate-buffered saline

Pen-Strep

Penicillin-Streptomycin

RPR

Rapid plasma reagin

RT-PCR

Reverse transcription polymerase chain reaction

SOP

Standard operating procedure

TSE

Transmissible spongiform encephalopathy

Notes

Acknowledgments

The authors would like to thank Dr. Mohsen Khorshidi, Shokouh Salimi, Maryam Afshari, and Ali Valadkhani for their considerable assistance.

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Parisa Goodarzi
    • 1
  • Hamid Reza Aghayan
    • 2
  • Moloud Payab
    • 3
  • Bagher Larijani
    • 4
  • Sepideh Alavi-Moghadam
    • 5
  • Masoumeh Sarvari
    • 2
  • Hossein Adibi
    • 6
  • Fatemeh Khatami
    • 7
  • Najmeh Foroughi Heravani
    • 2
  • Mahdieh Hadavandkhani
    • 2
  • Babak Arjmand
    • 2
    • 5
    Email author
  1. 1.Brain and Spinal Cord Injury Research CenterNeuroscience Institute, Tehran University of Medical SciencesTehranIran
  2. 2.Cell Therapy and Regenerative Medicine Research CenterEndocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical SciencesTehranIran
  3. 3.Obesity and Eating Habits Research CenterEndocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical SciencesTehranIran
  4. 4.Endocrinology and Metabolism Research CenterEndocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical sciencesTehranIran
  5. 5.Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical SciencesTehranIran
  6. 6.Diabetes Research CenterEndocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical SciencesTehranIran
  7. 7.Chronic Diseases Research CenterEndocrinology and Metabolism Population Sciences Institute, Tehran University of Medical SciencesTehranIran

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