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Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling

  • Xuan Su
  • Yan Shen
  • Neal L. Weintraub
  • Yaoliang TangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2150)

Abstract

Stem cell-based therapies hold great promise as alternative therapeutic strategies for various chronic diseases, including ischemic cardiomyopathy. Tracking the engraftment of transplanted stem cells is critical to the assessment of donor cell survival in the host environment. Fluorescent proteins, such as green fluorescent protein (GFP), have been widely used to track the fate of donor cells; however, GFP labeling has limitations with regard to noninvasively measuring cell engraftment in vivo. Our research indicates that near-infrared fluorescent protein (iRFP) labeling offers advantages for noninvasive in vivo imaging and histological assessment. Here, we present a protocol for using the lentiviral vector-mediated iRFP vector system to label and track donor stem cells in ischemic mouse hearts.

Keywords

Stem cells iRFP Green fluorescent protein (GFP) Lentivirus Myocardial infarction 

Notes

Acknowledgments

Weintraub and Y. Tang were partially supported by the American Heart Association: GRNT31430008, NIH-AR070029, NIH-HL086555, and NIH-HL134354. All authors declare that he/she has no conflict of interest. This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Xuan Su
    • 1
  • Yan Shen
    • 1
  • Neal L. Weintraub
    • 1
  • Yaoliang Tang
    • 1
    Email author
  1. 1.Vascular Biology Center, Medical College of GeorgiaAugusta UniversityAugustaUSA

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