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Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry

  • Rasmus Jentoft Boutrup
Protocol
Part of the Methods in Molecular Biology book series

Abstract

Immunohistochemistry (IHC) is a frequently used technique in life science and in clinic diagnostic. IHC is a high precision method to localize different cell types or their expression in tissue. Over the years, different approaches of IHC have emerged, and the technique has become more and more sophisticated. However, the principles still remains: the inherent and spontaneous non-covalent interaction between an antibody and (hypothetical) any target of interest. That means, using this technique allows you to analyze a wide range of histological tissues (muscles, organs, neurons, etc.) from humans or animals under the microscope. Literally, IHC makes the invisible to the human eye clearly visible. In this chapter, we present an approach how to analyze human skeletal muscle tissue for content and activity of muscle stem cells, termed satellite cells.

Keywords

MeSH terms Immunohistochemistry Antibodies Antigens Stem cells Muscles 

Abbreviations

BSA

Bovine serum albumin

DAPI

4′,6-Diamidino-2-phenylindole

FBS

Fetal bovine serum

GS

Goat serum

Pax7

Paired box protein-7

SC

Satellite cell

PBS

Phosphate buffered saline

MHC-I

Myosin heavy chain I

nMHC

Neonatal myosin heavy chain

Notes

Acknowledgments

I would like to thank Jean Farup for introducing me to the amazing world of immunohistochemistry and for sharing all his great knowledge about muscle and satellite cells. I would also thank my colleague on VIA University College, Malene Munk Jørgensen, for her critical review of this manuscript.

References

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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Rasmus Jentoft Boutrup
    • 1
  1. 1.Section for Sport Science, Department of Public HealthAarhus UniversityAarhus CDenmark

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