pp 1-10 | Cite as

Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry

  • Rasmus Jentoft Boutrup
Part of the Methods in Molecular Biology book series


Immunohistochemistry (IHC) is a frequently used technique in life science and in clinic diagnostic. IHC is a high precision method to localize different cell types or their expression in tissue. Over the years, different approaches of IHC have emerged, and the technique has become more and more sophisticated. However, the principles still remains: the inherent and spontaneous non-covalent interaction between an antibody and (hypothetical) any target of interest. That means, using this technique allows you to analyze a wide range of histological tissues (muscles, organs, neurons, etc.) from humans or animals under the microscope. Literally, IHC makes the invisible to the human eye clearly visible. In this chapter, we present an approach how to analyze human skeletal muscle tissue for content and activity of muscle stem cells, termed satellite cells.


MeSH terms Immunohistochemistry Antibodies Antigens Stem cells Muscles 



Bovine serum albumin




Fetal bovine serum


Goat serum


Paired box protein-7


Satellite cell


Phosphate buffered saline


Myosin heavy chain I


Neonatal myosin heavy chain



I would like to thank Jean Farup for introducing me to the amazing world of immunohistochemistry and for sharing all his great knowledge about muscle and satellite cells. I would also thank my colleague on VIA University College, Malene Munk Jørgensen, for her critical review of this manuscript.


  1. 1.
    Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M (2012) Applications of immunohistochemistry. J Pharm Bioallied Sci 4(Suppl 2):S307–S309. Scholar
  2. 2.
    Boutrup RJ, Farup J, Vissing K, Kjaer M, Mikkelsen UR (2018) Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study. Rheumatol Int 38(6):1031–1041. Scholar
  3. 3.
    Kalyuzhny AE (2016) Immunohistochemistry. Essential elements and beyond, vol 36(6), Springer International, Cham, p 3226. doi: Scholar
  4. 4.
    Ramos-Vara JA, Miller MA (2014) When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry—the red, brown, and blue technique. Vet Pathol 51(1):42–87. Scholar

Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Rasmus Jentoft Boutrup
    • 1
  1. 1.Section for Sport Science, Department of Public HealthAarhus UniversityAarhus CDenmark

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