Total Nitrogen Determination by a Spectrophotometric Method

  • Jaana Koistinen
  • Mervi Sjöblom
  • Kristian Spilling
Part of the Methods in Molecular Biology book series (MIMB, volume 1980)


Being able to measure total nitrogen (TN) is important for following the nitrogen budget. In this chapter, we present the spectrophotometric method we use for determining TN. The method relies on oxidation and reduction steps, involving persulfate digestion of nitrogen compounds into nitrate followed by spectrophotometric determination.


Nutrient uptake Total nitrogen 


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Copyright information

© Springer Science+Business Media New York 2019

Authors and Affiliations

  • Jaana Koistinen
    • 1
  • Mervi Sjöblom
    • 1
  • Kristian Spilling
    • 2
  1. 1.Tvärminne Zoological StationUniversity of HelsinkiHankoFinland
  2. 2.Finnish Environment InstituteHelsinkiFinland

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