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Analysis of Hematopoietic Niche in the Mouse Embryo

  • Keai Sinn Tan
  • Nathalie Brouard
  • Daisuke SugiyamaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2002)

Abstract

The development, differentiation, and maturation of hematopoietic cells are regulated by the intrinsic and extrinsic regulation. Intrinsic activity is affected by cell autonomous gene expression and extrinsic factors originate from the so-called niche surrounding the hematopoietic cells. It remains unclear why the hematopoietic sites are shifted during embryogenesis. Flow cytometry and immunohistochemistry enable us to study embryonic regulation of hematopoietic niche in the mouse embryo.

Keywords

Embryo Hematopoietic Mouse Niche 

Notes

Acknowledgments

We thank the Ministry of Education, Culture, Sports, Science and Technology; The Ministry of Health, Labor and Welfare; and the Japan Society for the Promotion of Science for grant support.

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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Keai Sinn Tan
    • 1
  • Nathalie Brouard
    • 2
  • Daisuke Sugiyama
    • 3
    • 4
    • 5
    • 6
    • 7
    Email author
  1. 1.Institute of Biomedical and Pharmaceutical SciencesGuangdong University of TechnologyGuangzhouChina
  2. 2.Unité Mixte de Recherche - S1255 Recherche Plaquettes Sanguines: Hémostase, Thrombose, TransfusionUniversité de Strasbourg, INSERM, Etablissement Français du Sang-Alsace, Fédération de Médecine Translationnelle de StrasbourgStrasbourgFrance
  3. 3.Department of Research and Development of Next Generation Medicine, Faculty of Medical SciencesKyushu UniversityFukuokaJapan
  4. 4.Center for Clinical and Translational ResearchKyushu University HospitalFukuokaJapan
  5. 5.Incubation Center for Advanced Medical ScienceKyushu UniversityFukuokaJapan
  6. 6.Department of Clinical Study, Center for Advanced Medical InnovationKyushu UniversityFukuokaJapan
  7. 7.Kyushu University, Station for Collaborative Research1 4FFukuokaJapan

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