Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

  • Ehsan Taghiabadi
  • Bahareh Beiki
  • Nasser Aghdami
  • Amir BajouriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1879)


Application of cell-based skin substitutes has recently evolved as a novel treatment for hard-to-heal wounds. Here, we focus on the development of a novel skin substitute by seeding human adipose-derived stromal cells (ASCs) on acellular human amniotic membrane (HAM). This construction is probably associated with higher rates of host cell infiltration and implanted cell engraftment. ASCs are achieved by separation of stromal cells from lipoaspirates using collagenase digestion and acellular HAM was obtained by separation of outer membrane of the chorion and removing its epithelial cells.


Adipose-derived stromal cells Amniotic membrane Skin substitutes Stem cell Tissue engineering Wound healing 



The protocols described here were developed with the support of Royan Institute grant.


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Ehsan Taghiabadi
    • 1
    • 2
  • Bahareh Beiki
    • 2
  • Nasser Aghdami
    • 1
  • Amir Bajouri
    • 1
    Email author
  1. 1.Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and TechnologyACECRTehranIran
  2. 2.Skin and Stem Cell Research CenterTehran University of Medical SciencesTehranIran

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