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Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells
Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.
KeywordsHematopoietic stem cell MACS Skin stem cell Spermatogonial stem cell
The experiments presented in this protocol chapter were supported by grants from Technical and Research Council of Turkey (#113S819) and Hacettepe University Research Funds (#013D04101005, #THD-2017-13430).
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