Melanoblasts as Multipotent Cells in Murine Skin
Melanoblasts (MBs) are melanocyte precursors that are derived from neural crest cells (NCCs). We recently demonstrated the multipotency of MBs; they differentiate not only into pigmented melanocytes but also other NCC derivatives. We herein describe methods for the isolation of MBs from mouse skin by flow cytometry. Methods to culture isolated MBs that retain their multipotency and isolation methods for MBs using gene-modified mouse are also described.
KeywordsCD45 Flow cytometer KIT Melanoblasts Multipotency SOX10
This study was supported by a grant from the program Grants-in-Aid for Scientific Research (C) from the Japan Society for Promotion of Science.