Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

  • Ehsan Taghiabadi
  • Bahareh Beiki
  • Nasser Aghdami
  • Amir BajouriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1879)


Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.


Amniotic membrane Fetal fibroblast Skin substitutes Stem cell Tissue engineering Wound healing 



The protocols described here were developed under the support of a Royan institute grant.


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Ehsan Taghiabadi
    • 1
    • 2
  • Bahareh Beiki
    • 2
  • Nasser Aghdami
    • 1
  • Amir Bajouri
    • 1
    Email author
  1. 1.Department of Regenerative Biomedicine, Cell Science Research CenterRoyan Institute for Stem Cell Biology and Technology, ACECRTehranIran
  2. 2.Skin and Stem Cell Research CenterTehran University of Medical SciencesTehranIran

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