Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration
Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11–14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.
KeywordsAmniotic membrane Fetal fibroblast Skin substitutes Stem cell Tissue engineering Wound healing
The protocols described here were developed under the support of a Royan institute grant.
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