pp 1-10 | Cite as

Determining Inorganic and Organic Nitrogen

  • Jaana KoistinenEmail author
  • Mervi Sjöblom
  • Kristian Spilling
Part of the Methods in Molecular Biology book series


Nitrogen (N) is one of the key nutrients for algal growth and is an integral part of many cellular components, for example in proteins. Being able to determine the inorganic and organic pools of N is consequently critical for algal cultivation. In this chapter we present the methods we use for determining dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON). The methods presented here for DIN rely on colorimetric methods and those of DON and PON on filtration and high temperature catalytic oxidation.


Ammonium Dissolved organic nitrogen Nitrate Nutrient uptake Particulate organic nitrogen 


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Copyright information

© Springer Science+Business Media New York 2018

Authors and Affiliations

  • Jaana Koistinen
    • 1
    Email author
  • Mervi Sjöblom
    • 1
  • Kristian Spilling
    • 2
  1. 1.Tvärminne Zoological StationUniversity of HelsinkiHankoFinland
  2. 2.Finnish Environment InstituteHelsinkiFinland

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