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Organoids pp 13-22 | Cite as

Clinically Amendable, Defined, and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells

  • Eva Tomaskovic-CrookEmail author
  • Jeremy M. CrookEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1576)

Abstract

Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined, and reliable for reproducible and scalable organoid generation, and amendable to principles of current good laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing practice (cGMP) toward clinical compliance.

Keywords

Brain organoid Clinical compliance Defined GelMA Human induced pluripotent stem cells Hydrogel 

Notes

Acknowledgment

The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation CampusUniversity of WollongongWollongongAustralia
  2. 2.Illawarra Health and Medical Research InstituteUniversity of WollongongWollongongAustralia
  3. 3.Department of Surgery, St Vincent’s HospitalThe University of MelbourneFitzroyAustralia

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