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Organoids pp 195-204 | Cite as

The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism

  • Diana Li
  • Hongli Dong
  • Alison B. KohanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1576)

Abstract

Since the initial report in 2009 by Sato and Clevers, primary enteroids have been of major interest in the fields of stem cell biology and gastrointestinal (GI) tract biology. More recently, we and others have made major inroads into the physiological relevance of these enteroid models and have shown that enteroids derived from the stomach, intestine, or colon recapitulate major functions of these tissues, namely, gastric acid secretion, lipid absorption and lipoprotein secretion, and ion transport. Here, we detail the isolation of stem cells from the small intestine and the culture and propagation of those stem cells into mature three-dimensional enteroids. We will also detail how we use enteroids to determine intestinal mechanisms behind dietary lipid absorption and lipoprotein secretion. The primary enteroid model is a powerful tool that significantly expands our ability to model GI tract function in vitro.

Keywords

Chylomicron Enteroids Intestinal stem cell Lipid absorption Model of small intestine Organoids Primary enterocytes 

Abbreviations

apo

Apolipoprotein

BSA

Bovine serum albumin

FFA

Free fatty acids

ISCs

Intestinal stem cells

LGR5

Leucine-rich repeat-containing G-protein-coupled receptor 5

MG

Monoacylglycerol

OA

Oleic acid

TAG

Triacylglycerol

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Nutritional SciencesUniversity of ConnecticutStorrsUSA

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