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Autophagy in Adipocyte Differentiation

  • Zhipeng Tao
  • Longhua Liu
  • Louise D. Zheng
  • Zhiyong Cheng
Part of the Methods in Molecular Biology book series (MIMB, volume 1854)

Abstract

Adipose tissue regulates metabolic homeostasis by acting as an endocrine organ and energy reservoir. Adipose tissue development and functional maintenance are dependent on adipocyte differentiation, in which autophagy plays an important role. It has been shown that autophagy deficiency dampens adipocyte differentiation, compromises adipose tissue development, dysregulates adipocytokine secretion, and even causes sudden death in young animals. Therefore, accurate assessment of autophagy in adipocyte is critical for the study of adipose biology or pathology of metabolic diseases. In this chapter, we described the procedure of autophagy analysis during adipocyte differentiation, and discussed the power of steady-state autophagy protein (e.g., beclin 1, LC3, and p62) levels versus autophagy flux to reflect autophagy activity.

Keywords

Adipocyte differentiation Adipose development and function Autophagy Endocrine Metabolism 

Notes

Acknowledgment

This work was supported in part by USDA National Institute of Food and Agriculture Hatch Project 1007334 (ZC).

References

  1. 1.
    Kershaw EE, Flier JS (2004) Adipose tissue as an endocrine organ. J Clin Endocrinol Metab 89:2548–2556CrossRefPubMedGoogle Scholar
  2. 2.
    Galic S, Oakhill JS, Steinberg GR (2010) Adipose tissue as an endocrine organ. Mol Cell Endocrinol 316:129–139CrossRefPubMedGoogle Scholar
  3. 3.
    Slutsky N, Vatarescu M, Haim Y et al (2016) Decreased adiponectin links elevated adipose tissue autophagy with adipocyte endocrine dysfunction in obesity. Int J Obes (Lond) 40:912–920CrossRefGoogle Scholar
  4. 4.
    Rutkowski JM, Stern JH, Scherer PE (2015) The cell biology of fat expansion. J Cell Biol 208:501–512CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Crewe C, An YA, Scherer PE (2017) The ominous triad of adipose tissue dysfunction: inflammation, fibrosis, and impaired angiogenesis. J Clin Invest 127:74–82CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Mizushima N, Komatsu M (2011) Autophagy: renovation of cells and tissues. Cell 147:728–741CrossRefGoogle Scholar
  7. 7.
    Green DR, Levine B (2014) To be or not to be? How selective autophagy and cell death govern cell fate. Cell 157:65–75CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Liu L, Tao Z, Zheng LD et al (2016) FoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes. Cell Death Dis 2:16066CrossRefGoogle Scholar
  9. 9.
    Liu L, Zheng LD, Zou P et al (2016) FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes. Cell Cycle 15:2033–2041CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Singh R, Xiang Y, Wang Y et al (2009) Autophagy regulates adipose mass and differentiation in mice. J Clin Invest 119:3329–3339CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Zhang Y, Goldman S, Baerga R, Zhao Y, Komatsu M, Jin S (2009) Adipose-specific deletion of autophagy-related gene 7 (atg7) in mice reveals a role in adipogenesis. Proc Natl Acad Sci U S A 106:19860–19865CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Mizushima N, Yoshimori T, Levine B (2010) Methods in mammalian autophagy research. Cell 140:313–326CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Klionsky DJ, Abdelmohsen K, Abe A et al (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12:1–222CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Liu L, Zheng LD, Donnelly SR, Emont MP, Wu J, Cheng Z (2017) Isolation of mouse stromal vascular cells for monolayer culture. Methods Mol Biol 1566:9–16CrossRefPubMedGoogle Scholar
  15. 15.
    Liu L, Zou P, Zheng L et al (2015) Tamoxifen reduces fat mass by boosting reactive oxygen species. Cell Death Dis 6:e1586CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Zou P, Liu L, Zheng L et al (2014) Targeting FoxO1 with AS1842856 suppresses adipogenesis. Cell Cycle 13:3759–3767CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Zhipeng Tao
    • 1
  • Longhua Liu
    • 1
    • 2
  • Louise D. Zheng
    • 1
  • Zhiyong Cheng
    • 1
  1. 1.Department of Human Nutrition, Foods, and ExerciseFralin Translational Obesity Research Center, College of Agriculture and Life ScienceBlacksburgUSA
  2. 2.Department of Pathology and Cell Biology, Naomi Berrie Diabetes CenterCollege of Physicians and Surgeons, Columbia UniversityNew YorkUSA

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