Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds.
Bone tissue engineering 3D-printing Decellularized bone matrix Polycaprolactone Adipose-derived stem cells
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Elder M (2014) Advanced orthopedic technologies, implants and regenerative products. BCC Research, Wellesley, MAGoogle Scholar
Gainer K (2015) Regenerative medicines: bone and joint applications. BCC Research, Wellesley, MAGoogle Scholar
Temple JP, Hutton DL, Hung BP et al (2014) Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds. J Biomed Mater Res A 102:4317–4325PubMedGoogle Scholar
Williams JM, Adewunmi A, Schek RM et al (2005) Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. Biomaterials 26:4817–4827CrossRefPubMedGoogle Scholar
Shor L, Güçeri S, Wen X et al (2007) Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro. Biomaterials 28:5291–5297CrossRefPubMedGoogle Scholar
Woodruff MA, Hutmacher DW (2010) The return of a forgotten polymer—polycaprolactone in the 21st century. Prog Polym Sci 35:1217–1256CrossRefGoogle Scholar
García-Gareta E, Coathup MJ, Blunn GW (2015) Osteoinduction of bone grafting materials for bone repair and regeneration. Bone 81:112–121CrossRefPubMedGoogle Scholar
Penel G, Delfosse C, Descamps M et al (2005) Composition of bone and apatitic biomaterials as revealed by intravital Raman microspectroscopy. Bone 36:893–901CrossRefPubMedGoogle Scholar
Benders KEM, van Weeren PR, Badylak SF et al (2013) Extracellular matrix scaffolds for cartilage and bone regeneration. Trends Biotechnol 31:169–176CrossRefPubMedGoogle Scholar
Datta N, Holtorf HL, Sikavitsas VI et al (2005) Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells. Biomaterials 26:971–977CrossRefPubMedGoogle Scholar
Nyberg E, Rindone A, Dorafshar A et al (2016) Comparison of 3D-printed poly-ε-caprolactone scaffolds functionalized with tricalcium phosphate, hydroxyapatite, bio-Oss, or decellularized bone matrix. Tissue Eng Part A. doi:10.1089/ten.TEA.2016.0418Google Scholar