Organoids pp 1-12 | Cite as

A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment

  • Magdalena Sutcliffe
  • Madeline A. LancasterEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1576)


3D brain organoids are a powerful tool with prospective application for the study of neural development and disease. Here we describe the growth factor-free method of generating cerebral organoids from feeder-dependent or feeder-free human pluripotent stem cells using standard laboratory equipment. The protocol outlined below allows generation of 3D tissues, which replicate human early in vivo brain development up to the end of the first trimester, both in terms of morphology and gene expression pattern.


Brain In vitro Neural differentiation Neurobiology Neurodevelopment Organoids Stem cells Three-dimensional 



We would like to thank all members of the Lancaster Lab for helpful discussion. Research in the Lancaster Lab is supported by the MRC (MC_UP_1201/9) as well as a 3Rs prize from the NC3Rs.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.MRC Laboratory of Molecular BiologyCambridgeUK

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