3D brain organoids are a powerful tool with prospective application for the study of neural development and disease. Here we describe the growth factor-free method of generating cerebral organoids from feeder-dependent or feeder-free human pluripotent stem cells using standard laboratory equipment. The protocol outlined below allows generation of 3D tissues, which replicate human early in vivo brain development up to the end of the first trimester, both in terms of morphology and gene expression pattern.
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We would like to thank all members of the Lancaster Lab for helpful discussion. Research in the Lancaster Lab is supported by the MRC (MC_UP_1201/9) as well as a 3Rs prize from the NC3Rs.
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