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Organoids pp 205-213 | Cite as

Oncogenic Transformation of Human-Derived Gastric Organoids

  • Nina Bertaux-Skeirik
  • Jomaris Centeno
  • Jian Gao
  • Joel Gabre
  • Yana ZavrosEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1576)

Abstract

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

Keywords:

Human fundic gastric organoids CRISPR/Cas-9 Nucleofection Organoid orthotopic transplantation 

Notes

Acknowledgements

This work was supported by NIH 1R01DK083402 grant and College of Medicine Bridge Funding Program (Zavros), NIH 5T32GM105526, Albert J. Ryan Fellowship, and Dean’s Fellowship (Bertaux-Skeirik).

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© Springer Science+Business Media New York 2016

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Authors and Affiliations

  • Nina Bertaux-Skeirik
    • 1
  • Jomaris Centeno
    • 2
  • Jian Gao
    • 3
  • Joel Gabre
    • 4
  • Yana Zavros
    • 5
    Email author
  1. 1.Department of Molecular and Cellular PhysiologyUniversity of Cincinnati College of MedicineCincinnatiUSA
  2. 2.Biology DepartmentUniversity of Puerto Rico-Río PiedrasSan JuanPuerto Rico
  3. 3.Department of PediatricsW.F. Maternal & Child Health HospitalWeifangPeople’s Republic of China
  4. 4.Department of Internal MedicineUniversity of CincinnatiCincinnatiUSA
  5. 5.Department of Molecular and Cellular PhysiologyUniversity of Cincinnati College of MedicineCincinnatiUSA

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