Oncogenic Transformation of Human-Derived Gastric Organoids
The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.
Keywords:Human fundic gastric organoids CRISPR/Cas-9 Nucleofection Organoid orthotopic transplantation
This work was supported by NIH 1R01DK083402 grant and College of Medicine Bridge Funding Program (Zavros), NIH 5T32GM105526, Albert J. Ryan Fellowship, and Dean’s Fellowship (Bertaux-Skeirik).
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