Organoid Culture of Human Cancer Stem Cells
Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.
Keywords:Organoids Cancer stem cells Clonogenicity Matrigel Growth factor cocktails Passage
We thank the divisions of Breast and Endocrine Surgery and of Gastrointestinal Surgery of Kobe University Graduate School of Medicine, and the division of Surgery of Nagoya University for contribution in the patient tumor collection.
This study was supported by Grants-in-Aid for Scientific Research from the Japan Society of the Promotion of Science and by the grants from Japan Foundation for Applied Enzymology and the Itoh-Chubei Foundation to Y.S.
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