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Organoids pp 55-92 | Cite as

Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells

  • Mahboobe GhaediEmail author
  • Laura E. Niklason
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1576)

Abstract

Induced pluripotent stem (iPS) cells are the product of adult somatic cell reprogramming to an embryonic-like state by inducing a “forced” expression of specific genes. They are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in many aspects, such as the expression of certain stem cell genes and potency and differentiability. Human iPS cells are invaluable resource for basic research, cell therapy, drug discovery, and human organ tissue engineering. iPS cells can be derived from the patient to be treated and thus are genetically identical cells that may avoid immune rejection. The following protocols offer a general guideline for the induction of iPSCs from fibroblasts, and for culture and expansion to produce lung precursor cells.

Keywords:

Human induced pluripotent stem cell Reprogramming Expansion Cell culture Differentiation Progenitors 

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© Springer Science+Business Media New York 2016

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Authors and Affiliations

  1. 1.Departments of Anesthesia and Biomedical EngineeringYale UniversityNew HavenUSA
  2. 2.Department of Biomedical EngineeringYale UniversityNew HavenUSA

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