Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells
Induced pluripotent stem (iPS) cells are the product of adult somatic cell reprogramming to an embryonic-like state by inducing a “forced” expression of specific genes. They are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in many aspects, such as the expression of certain stem cell genes and potency and differentiability. Human iPS cells are invaluable resource for basic research, cell therapy, drug discovery, and human organ tissue engineering. iPS cells can be derived from the patient to be treated and thus are genetically identical cells that may avoid immune rejection. The following protocols offer a general guideline for the induction of iPSCs from fibroblasts, and for culture and expansion to produce lung precursor cells.
Keywords:Human induced pluripotent stem cell Reprogramming Expansion Cell culture Differentiation Progenitors
- 2.BD Biosciences. (2013) Assay methods protocol: human embryonic stem cell culture. https://www.daigger.com:1-7
- 10.Stem Cell Technologies. (2012) Technical manual: maintenance of human pluripotent stem cells in mTeSR™1 and TeSR™2. http://www.stemcell.com:1-24
- 12.Van Haute L, De Block G, Liebaers I et al. (2009) Generation of lung epithelial-like tissue from human embryonic stem cells. Respir Res 10:105Google Scholar
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