Abstract
Yersinia pestis can be identified in the laboratory by conventional bacteriologic test; however, the process is lengthy (at least 2 days). Alternatively, immunological techniques can rapidly detect and identify the organism. This chapter describes methods for Y. pestis identification by immunological methods, including reverse indirect hemagglutination assay (RIHA), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), and immunological biosensor technique.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Prentice MB, Rahalison L (2007) Plague. Lancet 369(9568):1196–1207
Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 10(1):35–66
Yan Z, Zhou L, Zhao Y, Wang J, Huang L, Hu K, Liu H, Wang H, Guo Z, Song Y (2006) Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor. Sensors Actuators B Chem 119(2):656–663
Zhang P, Liu X, Wang C, Zhao Y, Hua F, Li C, Yang R, Zhou L (2014) Evaluation of up-converting phosphor technology-based lateral flow strips for rapid detection of Bacillus anthracis Spore, Brucella spp., and Yersinia pestis. PLoS One 9(8):e105305
Cao LK, Anderson GP, Ligler FS, Ezzell J (1995) Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor. J Clin Microbiol 33(2):336–341
Wei H, Zhao Y, Bi Y, Liu H, Guo Z, Song Y, Zhai J, Huang H, Yang R (2007) Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor. Sensors Actuators B Chem 123(1):204–210
Yang Y, Wang M, Zhao X, Zhao Z, Zhang A, Wei R, Wei B, Wang Z (2014) Analysis on the results of etiology and serology of plague in Qinghai province from 2001 to 2010. Zhonghua yu fang yi xue za zhi [Chinese J Prev Med] 48(2):124–127
Wang ZY, Dai RX, Wei BQ, Qi ZZ, Yang XY, Xin YQ, Zhang SH (2006) Plague diagnosis: reverse indirect hemagglutination assay versus bacteria culture. Chin J Endemiol 33(13):651–664
Lindsey CY, Powell BS, Bolt CR, Brown JE, Adamovicz JJ (2012) Validation of quantitative ELISAS for measuring anti-Yersinia pestis F1 and V antibody concentrations in nonhuman primate sera. J Immunoass Immunochem 33(1):91–113
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this protocol
Cite this protocol
Zhao, Y. (2018). Immunological Methods for Detection and Identification of Yersinia pestis . In: Yang, R. (eds) Yersinia Pestis Protocols. Springer Protocols Handbooks. Springer, Singapore. https://doi.org/10.1007/978-981-10-7947-4_29
Download citation
DOI: https://doi.org/10.1007/978-981-10-7947-4_29
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7946-7
Online ISBN: 978-981-10-7947-4
eBook Packages: Springer Protocols