Abstract
Gene regulation at the transcriptional level plays important roles in bacterial physiology, survival against stresses, and pathogenesis. In the previous studies, I employed a set of methods, including a microarray assay, an electrophoretic mobility shift assay, a DNase I footprinting assay, a primer extension assay, a LacZ fusion assay, and a real-time reverse transcription–polymerase chain reaction, to elucidate the molecular mechanisms of gene expression at the transcriptional level. These methods allow us to easily identify a transcriptional factor’s regulon and the transcriptional regulation mechanisms of target genes by a transcriptional factor. Here I describe detailed protocols of these methods.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Browning DF, Busby SJ (2004) The regulation of bacterial transcription initiation. Nat Rev Microbiol 2(1):57–65
Zhou D, Yang R (2006) Global analysis of gene transcription regulation in prokaryotes. Cell Mol Life Sci CMLS 63(19–20):2260–2290
Barnard A, Wolfe A, Busby S (2004) Regulation at complex bacterial promoters: how bacteria use different promoter organizations to produce different regulatory outcomes. Curr Opin Microbiol 7(2):102–108
Achtman M, Zurth K, Morelli G, Torrea G, Guiyoule A, Carniel E (1999) Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis. Proc Natl Acad Sci U S A 96(24):14043–14048
Perry RD (2003) A plague of fleas–survival and transmission of Yersinia pestis-this obligate parasite grows in fleas before being transmitted to mammalian hosts, part of a cycle essential for plague’s. ASM News-Am Soc Microbiol 69(7):336–340
Cornelis GR (2000) Molecular and cell biology aspects of plague. Proc Natl Acad Sci U S A 97(16):8778–8783
Lindler LE, Plano GV, Burland V, Mayhew GF, Blattner FR (1998) Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5 plasmid encoding murine toxin and capsular antigen. Infect Immun 66(12):5731–5742
Behr MA, Wilson MA, Gill WP, Salamon H, Schoolnik GK, Rane S, Small PM (1999) Comparative genomics of BCG vaccines by whole-genome DNA microarray. Science 284(5419):1520–1523
Zhou D, Han Y, Song Y, Tong Z, Wang J, Guo Z, Pei D, Pang X, Zhai J, Li M et al (2004) DNA microarray analysis of genome dynamics in Yersinia pestis: insights into bacterial genome microevolution and niche adaptation. J Bacteriol 186(15):5138–5146
Zhang Y, Gao H, Wang L, Xiao X, Tan Y, Guo Z, Zhou D, Yang R (2011) Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis. PLoS One 6(9):e25484
Andrews GP, Heath DG, Anderson GW Jr, Welkos SL, Friedlander AM (1996) Fraction 1 capsular antigen (F1) purification from Yersinia pestis CO92 and from an Escherichia coli recombinant strain and efficacy against lethal plague challenge. Infect Immun 64(6):2180–2187
Gao H, Zhang Y, Han Y, Yang L, Liu X, Guo Z, Tan Y, Huang X, Zhou D, Yang R (2011) Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis. BMC Microbiol 11:39
Gao H, Zhang Y, Yang L, Liu X, Guo Z, Tan Y, Han Y, Huang X, Zhou D, Yang R (2011) Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis. BMC Microbiol 11:40
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Nature Singapore Pte Ltd.
About this protocol
Cite this protocol
Zhang, Y. (2018). Genetic Regulation Analysis of Yersinia pestis . In: Yang, R. (eds) Yersinia Pestis Protocols. Springer Protocols Handbooks. Springer, Singapore. https://doi.org/10.1007/978-981-10-7947-4_22
Download citation
DOI: https://doi.org/10.1007/978-981-10-7947-4_22
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7946-7
Online ISBN: 978-981-10-7947-4
eBook Packages: Springer Protocols