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Cryopreservation

Conservation of Bioresources at Ultra Low Temperatures

  • Protocol
Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Summary

Cryopreservation is the ultra-low temperature storage (usually in liquid nitrogen at ca. -135 to -196°C) of living cells, tissues and organs capable of resuming normal functions after retrieval from a cryobank. This chapter explains the basic principles of cryopreservation with respect to strategies currently used to cryoprotect the diverse biological materials held in cryogenic storage. Approaches used to minimize or obviate the lethal and sublethal effects of cryoinjury are particularly highlighted. Examples of how cryopreservation has been universally applied to safeguard and preserve the wide spectrum of biological resources used in agriculture, biotechnology, healthcare, and biodiversity conservation are summarized and general guidance is offered for the management of bioresources in cryobanks.

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Acknowledgments

The authors acknowledge the European Union's 5th Framework Programme, Quality of Life and Management of Living Resources, Research Infrastructures Biological Collections, COBRA Project QLRT-2000-01645 and CRYMCEPT Project (QLK5-CT-2002-01279). J Nadarajan acknowledges the European Social Fund, The Forest Research Institute of Malaysia and the University of Abertay Dundee for the support of her postgraduate studies.

Author information

Authors and Affiliations

  1. Curator CCAP, Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Dunstaffnage Marine Laboratory, Dunbeg, Argyll, Scotland, UK

    John G. Day

  2. Environmental Science and Biotechnology, Cuparmuir, Cupar, Fife, Scotland, UK

    Keith C. Harding

  3. Millennium Seed Bank, Royal Botanic Gardens, Ardingly, Haywards Heath, W.Sussex, UK

    Jayanthi Nadarajan

  4. Environmental Science and Biotechnology, Cupar Muir, Cupar, Fife, Scotland, UK

    Erica E. Benson

Authors
  1. John G. Day
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  2. Keith C. Harding
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  3. Jayanthi Nadarajan
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  4. Erica E. Benson
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Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Hertfordshire, Hatfield, Hertfordshire, UK

    John M. Walker (Professor Emeritus) & Ralph Rapley (Professor Emeritus) & 

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Day, J.G., Harding, K.C., Nadarajan, J., Benson, E.E. (2008). Cryopreservation. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_52

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  • DOI: https://doi.org/10.1007/978-1-60327-375-6_52

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-370-1

  • Online ISBN: 978-1-60327-375-6

  • eBook Packages: Springer Protocols

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