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Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting

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The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Glycoproteins result from the covalent association of carbohydrate moieties (glycans) with proteins. The enzymatic glycosylation of proteins is a common and complex form of posttranslational modification. The precise roles played by the carbohydrate moieties of glycoproteins are beginning to be understood (1–3). It has been established that glycans perform important biological roles including: stabilization of the protein structure, protection from degradation, control of protein solubility, control of protein transport in cells, and control of protein half-life in blood. They also mediate the interactions with other macromolecules, and the recognition and association with viruses, enzymes, and lectins (4–6).

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Author information

Authors and Affiliations

  1. Clinical Research Unit, Psychiatric Institutions of Geneva, Switzerland

    Patricia Gravel

  2. Central Clinical Chemistry Laboratory, University Hospital of Geneva, Switzerland

    Olivier Golaz

Authors
  1. Patricia Gravel
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  2. Olivier Golaz
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1996 Humana Press Inc., Totowa, NJ

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Gravel, P., Golaz, O. (1996). Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_97

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_97

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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