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The Protein Protocols Handbook pp 467–472Cite as

Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate

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Abstract

The method described in this chapter is based on derivatization of amino acids, produced by hydrolysis of peptides or proteins, with phenylisothiocyanate. This forms phenylthiocarbamyl amino acids, which are then separated by reversed-phase high-performance liquid chromatography and quantified from their UV absorbance at 254 nm.

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References

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Author information

Authors and Affiliations

  1. School of Biology and Biochemistry, Queen’s University of Belfast, Ireland

    G. Brent Irvine

Authors
  1. G. Brent Irvine
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1996 Humana Press Inc., Totowa, NJ

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Irvine, G.B. (1996). Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_78

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_78

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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