Abstract
The heterobifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), is of value when one of the components of a conjugate has no free thiol groups, e.g., IgG. In this method (1), the IgG antibody is first modified by allowing the N-hydroxysuccinimide ester group of the MBS to react with amino groups in the IgG; the β-galactosidase is then added and the maleimide groups on the modified IgG react with thiol groups in the β-galactosidase to form thioether links. This procedure produces conjugates with molecular weights in the range 0.6–1 × 106.
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© 1996 Humana Press Inc., Totowa, NJ
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Wisdom, G.B. (1996). β-Galactosidase Labeling of Antibody Using MBS. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_42
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DOI: https://doi.org/10.1007/978-1-60327-259-9_42
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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