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The Protein Protocols Handbook pp 689–692Cite as

Conjugation of Peptide to Carrier Proteins via m-Maleimidobenzoyl-N-Hydroxysuccinimide Ester (MBS)

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Abstract

m-Maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) is a heterobifunctional agent that links a thiol group to an amino group at neutral pH. For peptide conjugation, the peptide usually provides the thiol group in the form of a cysteine residue, whereas the carrier provides amino groups in the form of lysine residues. The reaction proceeds in two steps. First the carrier is activated by reaction of its amino group with the succinimide moiety. Then the thiol group on the peptide reacts with the maleimide moiety of the activated carrier.

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References

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Author information

Authors and Affiliations

  1. Cytogen, Princeton, NJ

    J. Mark Carter

Authors
  1. J. Mark Carter
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1996 Humana Press Inc., Totowa, NJ

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Carter, J.M. (1996). Conjugation of Peptide to Carrier Proteins via m-Maleimidobenzoyl-N-Hydroxysuccinimide Ester (MBS). In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_118

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_118

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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