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N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)

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The Protein Protocols Handbook

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Abstract

The vast array of possible N-linked oligosaccharides demands high-resolution HPLC columns for their purification (1,2). Reverse-phase (C18) and normal-phase (NH2) columns have been used for the separation (singly or in concert) of many N-linked oligosaccharides. The porous graphitized carbon (PGC) column described in Chapter 106 for O-linked alditol separation will give improved N-linked oligosaccharide resolution over C18 columns, and has the advantage of using salt-free buffers for preparative work (3,4).

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References

  1. Hase, S. (1993) Analysis of sugar chains by pyridylamination, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 69–80.

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  2. Kakehi, K. and Honda, S. (1993) Analysis of carbohydrates in glycoproteins by high performance liquid chromatography and high performance capillary electrophoresis, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 81–98.

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  3. Smith, D. D., Davies, M. J., Hounsell, E. F. (1994) Structural profing of oligosaccharides of glycoproteins, in Methods in Molecular Biology, vol. 32: Basic Protein and Peptide Protocols (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 143–155.

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  4. Davies, M. J. and Hounsell, E. F. (1995) Comparison of separation modes for high performance liquid chromatography of glycoprotein-and proteoglycan-derived oligosaccharides. J. Chromatogr. 720, 227–234.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University College London, UK

    Elizabeth F. Hounsell & Michael J. Davies

  2. Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland, UK

    Kevin D. Smith

Authors
  1. Elizabeth F. Hounsell
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  2. Michael J. Davies
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  3. Kevin D. Smith
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1996 Humana Press Inc., Totowa, NJ

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Hounsell, E.F., Davies, M.J., Smith, K.D. (1996). N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC). In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_110

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_110

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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