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Mass Spectrometry

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Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Mass spectrometry (MS) (1) is one of the most important physical methods in analytical chemistry today A particular advantage of mass spectrometry, compared with other molecular spectroscopies, is its high sensitivity, so that it provides one of the few methods that is entirely suitable for the identification or quantitative measurement of trace amounts of samples.

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References

  1. Chapman, J R (1993) Practical Organic Mass Spectrometry A Guide for Chemical and Biochemical Analysis Wiley, Chichester, UK

    Google Scholar 

  2. Harrison, A G (1983) Chemicalionization Mass Spectrometry CRC, Boca Raton, FL

    Google Scholar 

  3. Barber, M, Bordoh, R S, Elliott, G J, Sedgwick, R D, and Tyler A N (1982) Fast atom bombardment mass spectrometry Anal Chem 54, 645A–657A

    Article  CAS  Google Scholar 

  4. Hillenkamp, F, Karas, M, Beavis, R C, and Chait, B T (1991) Matrix-assisted laser desorption/ionizationofbiopolymers Anal Chem 63, 1193A–1203A

    Article  PubMed  CAS  Google Scholar 

  5. Fenn, J B, Mann, M, Meng, C K, Wong, S F, and Whitehouse, C M (1990) Electrospray lomzation-principles and practice Mass Spectrom Rev 9, 37–70

    Article  CAS  Google Scholar 

  6. Dawson, P H (1976) Quadrupole Mass Spectrometry and its ApplicationsElsevier, NY

    Google Scholar 

  7. Duckworth, H E, Barber, R C, and Venkatasubramanian, V S(1988) Mass Spectroscopy, 2nd ed Cambridge University Press, Cambridge

    Google Scholar 

  8. Cotter, R J (1992) Time-of-flight mass spectrometry for the structural analysis of biological moleculesAnal Chem 64, 1027A–1039A

    Article  PubMed  CAS  Google Scholar 

  9. March, R E and Hughes, R J (1989) Quadrupole Storage Mass Spectrometry Wiley, New York

    Google Scholar 

  10. Asamoto, B (1991) FT-ICR MS Analytical Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry VCH, Weinheim

    Google Scholar 

  11. Busch, K L, Ghsh, G L, and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Techniques and Applications of Tandem Mass Spectrometry VCH, New York

    Google Scholar 

  12. He, S, Pan, S, Wu, K, Amster, I J, and Orlando, R (1995) Analysis of normal human fetal eye lens crystalline by high-performance liquid chromatography/mass spectrometry J Mass Spectrom 30,424–431

    Article  CAS  Google Scholar 

  13. Hogeland, K E,Jr and Deinzer, M L (1994) Mass spectrometnc studies on the N-linked ohgosaccharides of baculovirus-expressed mouse mterleukin-3 Biomed Environ Mass Spectrom 23,218–224

    Article  CAS  Google Scholar 

  14. Jacquinod, M, Halgand, F, Caffrey, M, Saint-Pierre, C, Gagnon, J, Fitch, J, Cusanovich, M, and Forest, E(1995) Conformational properties of Rhodobacter capsulatus cytochrome c2 wild-type and site-directed mutants using hydrogen/deuterium exchange monitored by electrospray lonization mass spectrometry Rapid Commun Mass Spectrom 9, 1135–1140

    Article  Google Scholar 

  15. Schwartz, B L, Gale, D C, and Smith, R D (1996) Noncovalent interactions observed using electrospray lonization, in Methods in Molecular Biology Protein and Peptide Analysis by Mass Spectromeny (Chapman, J R, ed), Humana, Totowa, NJ

    Google Scholar 

  16. Cottrell, J S and Sutton, C W (1996) The identification of electrophoretically separated proteins by peptide mass fingerprinting, in Methods in Molecular Biology Protein and Peptide Analysis by Mass Spectrometry (Chapman, J R, ed), Humana, Totowa, NJ

    Google Scholar 

  17. Naito, Y, Ishikawa, K, Koga, Y, Tsuneyoshi, T, Terunuma, H, and Arakawa, R (1995) Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray lonization mass spectrometry Rapid Commun Mass Spectrom 9, 1484–1486

    Article  PubMed  CAS  Google Scholar 

  18. Liu, Y-H, Bai, J, Zhu, Y, Liang, X, Siemieniak, D, yenta, P J, and Lubman, D M (1995) Rapid screening of genetic polymorphisms using buccal cell DNA with detection by matrix-assisted laser desorption/ionization mass spectrometry Rapid Commun Mass Spectrom 9, 735–743

    Article  PubMed  CAS  Google Scholar 

  19. Yang, Y and Orlando, R (1996) Identifying the glycosylation sites and site-specific carbohydrate heterogeneity of glycoproteins by matrix-assisted laser desorption/ionization mass spectrometry Rapid Commun Mass Spectrom 10, 932–936

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Cheshire, UK

    John R. Chapman

Authors
  1. John R. Chapman
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Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

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© 1998 Humana Press Inc , Totowa, NJ.

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Chapman, J.R. (1998). Mass Spectrometry. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_50

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  • DOI: https://doi.org/10.1007/978-1-59259-642-3_50

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

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