Abstract
To obtain information on the properties and structure of proteins, the use of specific probes is of great advantage in many cases. These probes may be, for example, substrate analogs (in case of enzymes) that firmly bind to the enzyme, or even suicide substrates that bind and inactivate the enzyme. Another group of very specific probes is represented by antibodies that specifically recognize structural parts of the protein. Over the last 15 years, the use of antibodies in detecting, characterizing, and purifying proteins has become a common method.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
Suggested Reading
R. A. Barneveld, F. P. W. Tegelaers, E. I. Ginns, P. Visser, E. A. Laanen, R. O. Brady, H. Galjaard, J. A. Barranger, A. J. J. Reuser, and J. M. Tager (1985) Monoclonal antibodies against β-glucocerebrosidase, Eur. J. Biochem. 134, 585–589.
C. A. Borrebaeck (1983) In vitro immunization for the production of antigen-specific lymphocyte hybridomas. Scand. J. Immunol. 18, 9–12.
A. M. Campbell (1984) Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas, in Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier, Amsterdam.
H. Hengartner, A. L. Luzzati, and M. Schreier (1978) Fusion of in vitro immobilized lymphoid cells with X63Ag8. Curr. Topics Microbial. Immunol. 81, 92–99.
J. Hilkens, J. M. Tager, F. Buijs, E. M. Brouwer-Kelder, G. M. Van Thienen, F. P. W. Tegelaers, and J. Hilgers (1981) Monoclonal antibodies against human acid α-glucosidase. Biochim. Biophys. Acta 678, 7–11.
K. Inaba, R. M. Steinman, W. C. Van Voorhis, and S. Muramatsu (1983) Dendritic cells are critical accessory cells for thymus-dependent antibody responses in mouse and man. Proc. Natl. Acad. Sci. USA 80, 6041–6045.
G. Kohler and C. Milstein (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495.
M. M. S. Lo, T. Y. Tsong, M. K. Conrad, S. M. Strittmatter, L. D. Hester, and S. H. Snijder (1984) Monoclonal antibody production by receptor mediated electrically induced cell fusion. Nature 310, 792–794.
R. A. Luben and M. A. Mohler (1980) In vitro immunization as an adjunct to the production of hybridomas producing antibodies against the lymphokine osteoclast activating factor. Mol. Immunol. 17, 635–639.
R. A. Luben, P. Brazeau, and R. Guillemin (1982) Monoclonal antibodies to hypothalamic growth hormone releasing factor with picomoles of antigen. Science 218, 887–889.
K. Lundgren, M. Wahlgren, M. Troye-Blomberg, K. Berzins, H. Perlmann, and P. Perlmann (1983) Monoclonal anti-parasite and anti-RBC antibodies produced by stable EBV-transformed B-cell lines from malaria patients. J. Immunol. 131, 2000–2003.
R. L. Pardue, R. C. Brady, G. W. Perry, and J. R. Dedman (1983) Production of monoclonal antibodies against calmodulin by in vitro immunization of spleen cells. J. Cell Biol. 96, 1149–1154.
C. L. Reading (1982) Theory and methods for immunization in culture and monoclonal antibody production. J. Immunol. Meth. 53, 261–291.
C. Stahli, V. Staehlin, J. Miggiano, J. Schmidt, and P. Haring (1980) High frequencies of antigen-specific hybridomas: Dependence on immunization parameters and prediction by spleen cell analysis. J. Immunol. Meth. 32, 297–304.
H. Towbin, T. Staehelin, and J. Gordon (1979) Electrophoretic transfer of proteins from polyacrylamind gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. USA 76, 4350.
J. Van Ness, U. K. Laemmli, and D. E. Pettijohn (1984) Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins. Proc. Natl. Acad. Sci. USA 81, 7897–7901.
D. M. Weir (1978) Handbook of Experimental Immunology, Blackwell, Oxford.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Humana Press Inc.
About this protocol
Cite this protocol
Schram, A.W. (1988). Use and Production of Polyclonal and Monoclonal Antibodies. In: Franks, F. (eds) Characterization of Proteins. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-437-5_15
Download citation
DOI: https://doi.org/10.1007/978-1-59259-437-5_15
Publisher Name: Humana Press
Print ISBN: 978-0-89603-109-8
Online ISBN: 978-1-59259-437-5
eBook Packages: Springer Book Archive