Abstract
Polyacrylamide gel electrophoresis (PAGE) is one of the most widely used techniques for the analytical separation of proteins and peptides. PAGE separations, based upon intrinsic charge-to-mass ratio (1), apparent molecular weight in the presence of sodium dodecyl sulfate (SDS) (2), and isoelectric focusing (3), all possess remarkable resolving power using amounts of protein ranging from a few picograms to several milligrams. When two of these techniques, molecular sieving with SDS and isoelectric focusing with ampholytes, are combined in two-dimensional separations (4), complex mixtures can be resolved into as many as several thousand components in a single experiment.
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© 1986 The Humana Press Inc.
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Hunkapiller, M.W., Lujan, E. (1986). Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_3
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DOI: https://doi.org/10.1007/978-1-59259-436-8_3
Publisher Name: Humana Press
Print ISBN: 978-0-89603-090-9
Online ISBN: 978-1-59259-436-8
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