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Applications of Visible Spectroscopy and Color Measurements in the Assessments of Carotenoid Levels in Foods

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Book cover Plant and Food Carotenoids

Abstract

The color of the food can be evaluated visually or instrumentally. The instrumental measurement allows to measure the color objectively and can be carried out by different techniques, most of them fast, cheap, and simple. Carotenoids are responsible for the color of many plants and foods. The measurement of color can be an effective tool to determine indirectly the concentration of carotenoids in plants and foods. In this chapter, we describe both traditional and recent methodologies for color measurement.

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References

  1. Wyszecki G, Stiles WS (1982) Color science. Concepts and methods. Quantitative data and formulae. John Wiley & Sons, New York

    Google Scholar 

  2. CIE (2004) Colorimetry, vol 142. CIE Central Bureau,Publ, Vienna, Austria, p 2001

    Google Scholar 

  3. Rodríguez-Concepcion M, Avalos J, Bonet ML et al (2018) A global perspective on carotenoids: metabolism, biotechnology, and benefits for nutrition and health. Prog Lipid Res 70:62–93

    Article  Google Scholar 

  4. Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2011) Applicability of the measurement of visible spectroscopic data and colour in the analysis of food carotenoid. In: Carotenoids: properties, effects, and diseases. Nova Science Publishers, Hauppauge, N.Y

    Google Scholar 

  5. Britton G, Liaaen-Jensen S, Pfander H (1995) Carotenoids. Volume 1B: Spectroscopy. Birkhäuser, Basel, Switzerland

    Google Scholar 

  6. Meléndez-Martínez AJ, Britton G, Vicario IM et al (2006) Relationship between the colour and the chemical structure of carotenoid pigments. Food Chem 101(3):1145–1150

    Article  Google Scholar 

  7. Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2017) Aplicación de las medidas de espectroscopía visible y de color al análisis de carotenoides. In: Carotenoides en agroalimentación y salud. Editorial Terracota SA, México, pp 118–140

    Google Scholar 

  8. Simon JE, Decker EA, Ferruzzi MG et al (2017) Establishing standards on colors from natural sources. J Food Sci 82(11):2539–2553

    Article  CAS  Google Scholar 

  9. Clydesdale FM (1993) Colour as a factor in food choice. Crit Rev Food Sci Nutr 33:83–101

    Article  CAS  Google Scholar 

  10. Ryan D, Robards K, Lavee S (1998) Assessment of quality in olive oil. Olivae 72:23–41

    Google Scholar 

  11. Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2005) Instrumental measurement of orange juice colour: a review. J Sci Food Agric 85(6):894–901. https://doi.org/10.1002/jsfa.2115

    Article  CAS  Google Scholar 

  12. Moyano MJ, Heredia FJ, Meléndez-Martínez AJ (2010) The color of olive oils: the pigments and their likely health benefits and visual and instrumental methods of analysis. Compr Rev Food Sci Food Saf 9(3):278–291

    Article  CAS  Google Scholar 

  13. Lehto S, Buchweitz M, Klimm A et al (2017) Comparison of food colour regulations in the EU and the US: a review of current provisions. Food Addit Contam Part A 34(3):335–355. https://www.tandfonline.com/. https://doi.org/10.1080/19440049.2016.1274431

    Article  CAS  Google Scholar 

  14. Martins N, Roriz CL, Morales P et al (2015) Food colorants: challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices. Trends Food Sci Technol 52:1–15

    Article  Google Scholar 

  15. Breithaupt DE (2007) Modern application of xanthophylls in animal feeding - a review. Trends Food Sci Technol 18(10):501–506. http://www.sciencedirect.com/science/article/B6VHY-4NKJ0D6-4/2/3c2f8dae3a7adbb884fb5d3ad9069a9f

    Article  CAS  Google Scholar 

  16. Breithaupt DE, Bamedi A, Wirt U (2002) Carotenol fatty acid esters: easy substrates for digestive enzymes? Comp Biochem Physiol Part B 132:721–728

    Article  Google Scholar 

  17. Breithaupt DE, Weller P, Grashorn MA (2003) Quantification of carotenoids in chicken plasma after feeding free or esterified lutein and capsanthin using high-performance liquid chromatography and liquid chromatography-mass spectrometry analysis. Poult Sci 82(3):395–401. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038548600&partnerID=40

    Article  CAS  Google Scholar 

  18. Maoka T (2011) Carotenoids in marine animals. Mar Drugs 9(2):278–293

    Article  CAS  Google Scholar 

  19. Shahidi F, Metusalach BJA et al (1998) Carotenoid pigments in seafoods and aquaculture. Crit Rev Food Sci Nutr 38(1):1–67

    Article  CAS  Google Scholar 

  20. Wade NM, Gabaudan J, Glencross BD (2017) A review of carotenoid utilisation and function in crustacean aquaculture. Rev Aquac 9(2):141–156

    Article  Google Scholar 

  21. de Carvalho CCR, Caramujo MJ (2017) Carotenoids in aquatic ecosystems and aquaculture: a colorful business with implications for human health. Front Mar Sci 4:93):1–93)14

    Article  Google Scholar 

  22. Spada FP, Selani MM, Coelho AAD et al (2016) Influence of natural and synthetic carotenoids on the color of egg yolk. Sci Agric 73(3):234–242

    Article  Google Scholar 

  23. Álvarez R, Meléndez-Martínez AJ, Vicario IM et al (2014) Effect of pasture and concentrate diets on concentrations of carotenoids, vitamin a and vitamin E in plasma and adipose tissue of lambs. J Food Compos Anal 36(1–2):59–65

    Article  Google Scholar 

  24. MacDougall D (2002) Colour in food, improving quality, 1st edn. Woodhead Publishing Ltd, Cambridge

    Book  Google Scholar 

  25. Hutchings JB (1994) Food colour and appearance. Blackie Academic and Professional Publication, Glasgow

    Book  Google Scholar 

  26. Wyszecki G, Stiles WS (2000) Color science. Concepts and methods. Quantitative data and formulae, vol 2. John Wiley & Sons, Inc, New York

    Google Scholar 

  27. Artigas JM (2002) Medida del color. In: Capilla P, Artigas JM, Pujol JE (eds) Fundamentos de colorimetría. Valencia, España

    Google Scholar 

  28. Workman J, Jerome J (2016) Ultraviolet spectroscopy. In: Workman J, Jerome J (eds) Concise handbook of analytical spectroscopy, the: theory, applications, and reference materials. World Scientific, Singapore

    Google Scholar 

  29. Skoog D, Holler FJ, Crouch SR et al (2008) Principios del Análisis Instrumental. Editorial Cengage Learning, México

    Google Scholar 

  30. Wang HH, Sun DW (2002) Correlation between cheese meltability determined with a computer vision method and with Arnott and Schreiber tests. J Food Sci 67:745–749

    Article  CAS  Google Scholar 

  31. Luo MR, Cui GH, Li C (2001) British patent entitled apparatus and method for measuring colour (DigiEye System). Application No 0124683.4. Derby University Enterprises Limited, 4 October

    Google Scholar 

  32. Heredia FJ, Álvarez C, González-Miret ML et al (2004) CromaLab, Analisis de color. Registro General de la Propiedad ́ Intelectual:2004

    Google Scholar 

  33. Stinco CM, Escudero-Gilete ML, Heredia FJ et al (2016) Multivariate analyses of a wide selection of orange varieties based on carotenoid contents, color and in vitro antioxidant capacity. Food Res Int 90:194–204

    Article  CAS  Google Scholar 

  34. Stinco CM, Heredia FJ, Vicario IM et al (2016) In vitro antioxidant capacity of tomato products: relationships with their lycopene, phytoene, phytofluene and alpha-tocopherol contents, evaluation of interactions and correlation with reflectance measurements. LWT-Food Sci Technol 65:718–724

    Article  CAS  Google Scholar 

  35. Meléndez-Martínez AJ, Gómez-Robledo L, Melgosa M et al (2011) Color of orange juices in relation to their carotenoid contents as assessed from different spectroscopic data. J Food Compos Anal 24(6):837–844

    Article  Google Scholar 

  36. Meléndez-Martínez AJ, Ayala F, Echávarri JF et al (2011) A novel and enhanced approach for the assessment of the total carotenoid content of foods based on multipoint spectroscopic measurements. Food Chem 126(4):1862–1869

    Article  Google Scholar 

  37. Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2007) Rapid assessment of vitamin a activity through objective color measurements for the quality control of orange juices with diverse carotenoid profiles. J Agric Food Chem 55(8):2808–2815

    Article  Google Scholar 

  38. Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2003) Application of tristimulus colorimetry to estimate the carotenoids content in ultrafrozen orange juices. J Agric Food Chem 51(25):7266–7270

    Article  Google Scholar 

  39. Stinco CM, Rodríguez-Pulido FJ, Escudero-Gilete ML et al (2013) Lycopene isomers in fresh and processed tomato products: correlations with instrumental color measurements by digital image analysis and spectroradiometry. Food Res Int 50(1):111–120. https://doi.org/10.1016/j.foodres.2012.10.011

    Article  CAS  Google Scholar 

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Correspondence to Antonio J. Meléndez-Martínez .

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Mapelli-Brahm, P., Rodríguez-Pulido, F.J., Stinco, C.M., Heredia, F.J., Meléndez-Martínez, A.J. (2020). Applications of Visible Spectroscopy and Color Measurements in the Assessments of Carotenoid Levels in Foods. In: Rodríguez-Concepción, M., Welsch, R. (eds) Plant and Food Carotenoids. Methods in Molecular Biology, vol 2083. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9952-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9952-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9951-4

  • Online ISBN: 978-1-4939-9952-1

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