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In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery

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Book cover Bioluminescent Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2081))

Abstract

Confirming the in vivo efficacy of potential antileishmanial compounds that display in vitro potency and good chemical characteristics is one of the most important steps in preclinical research drug discovery before human clinical trials begin. Here we describe the use of the in vivo bioluminescent monitoring of high and low inocula of luciferase-expressing Leishmania major (L. major) parasites in traditional and more innovative rodent models of in vivo cutaneous leishmaniasis (CL) drug discovery.

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Acknowledgments

Disclaimer: Material has been reviewed by the Walter Reed Army Institute of Research. There is no objection to its presentation and/or publication. The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense.

Research was conducted under an approved animal use protocol in an AAALACi accredited facility in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, NRC Publication, 2011 edition.

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Authors and Affiliations

  1. Walter Reed Army Institute of Research, Silver Spring, MD, USA

    Diana Caridha & Susan Leed

  2. Dwight D. Eisenhower Army Medical Center, Fort Gordon, GA, USA

    Alicia Cawlfield

Authors
  1. Diana Caridha
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  2. Susan Leed
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  3. Alicia Cawlfield
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Corresponding author

Correspondence to Diana Caridha .

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Editors and Affiliations

  1. The Center for Environmental Biotechnology, The University of Tennessee, 490 BioTech Inc., Knoxville, TN, USA

    Steven Ripp

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Caridha, D., Leed, S., Cawlfield, A. (2020). In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug Discovery. In: Ripp, S. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 2081. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9940-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9940-8_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9939-2

  • Online ISBN: 978-1-4939-9940-8

  • eBook Packages: Springer Protocols

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