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Protein Nanotechnology pp 73–84Cite as

ATP Synthase: Expression, Purification, and Function

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purification, and functional assessment of E. coli ATP synthase.

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Author information

Authors and Affiliations

  1. The Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia

    Meghna Sobti & Alastair G. Stewart

  2. University of Oxford, Oxford, UK

    Robert Ishmukhametov

Authors
  1. Meghna Sobti
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  2. Robert Ishmukhametov
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  3. Alastair G. Stewart
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Corresponding author

Correspondence to Alastair G. Stewart .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Sobti, M., Ishmukhametov, R., Stewart, A.G. (2020). ATP Synthase: Expression, Purification, and Function. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

  • eBook Packages: Springer Protocols

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