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Peroxiredoxin Proteins as Building Blocks for Nanotechnology

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

Peroxiredoxins are ubiquitous antioxidant proteins that exhibit a striking variety of quaternary structures, making them appealing building blocks with which nanoscale architectures are created for applications in nanotechnology. The solution environment of the protein, as well as protein sequence, influences the presentation of a particular structure, thereby enabling mesoscopic manipulations that affect arrangments at the nanoscale. This chapter will equip us with the knowledge necessary to not only produce and manipulate peroxiredoxin proteins into desired structures but also to characterize the different structures using dynamic light scattering, analytical centrifugation, and negative stain transmission electron microscopy, thereby setting the stage for us to use these proteins for applications in nanotechnology.

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Author information

Authors and Affiliations

  1. School of Biological Sciences, University of Auckland, Auckland, New Zealand

    Frankie Conroy

  2. Bio-Organic Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands

    N. Amy Yewdall

Authors
  1. Frankie Conroy
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  2. N. Amy Yewdall
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Corresponding author

Correspondence to N. Amy Yewdall .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Conroy, F., Yewdall, N.A. (2020). Peroxiredoxin Proteins as Building Blocks for Nanotechnology. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

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