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Protein Nanotechnology pp 17–37Cite as

Protein Expression in the Baculovirus-Insect Cell Expression System

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

The baculovirus-insect cell expression system is a popular choice for recombinant protein production. Post-translational modifications, production of protein complexes, and reported high protein yields are some of the favorable features of this eukaryotic expression system. The intricacies of the baculovirus-insect cell expression system may deter beginners from implementing it for routine protein production. This chapter documents a detailed protocol for using the baculovirus-insect cell expression system, which has been verified in a protein production research facility.

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References

  1. Jarvis DL (2009) Chapter 14 baculovirus–insect cell expression systems. In: Burgess RR, Deutscher MP (eds) Methods in enzymology, vol 463. Academic Press, San Diego, pp 191–222. https://doi.org/10.1016/S0076-6879(09)63014-7

    Chapter  Google Scholar 

  2. Tomlinson S, Ueda E, Maruniak JE, Garciacanedo A, Bjes ES, Esser AF (1993) The expression of hemolytically active human complement protein C9 in mammalian, insect, and yeast cells. Protein Expr Purif 4(2):141–148. https://doi.org/10.1006/prep.1993.1020

    Article  CAS  PubMed  Google Scholar 

  3. McCall EJ, Danielsson A, Hardern IM, Dartsch C, Hicks R, Wahlberg JM, Abbott WM (2005) Improvements to the throughput of recombinant protein expression in the baculovirus/insect cell system. Protein Expr Purif 42(1):29–36. https://doi.org/10.1016/j.pep.2005.03.021

    Article  CAS  PubMed  Google Scholar 

  4. Siew A (2016) Large-scale protein expression in baculovirus-infected insect cells. Biopharm Int 29(12):28–29

    CAS  Google Scholar 

  5. López-Vidal J, Gómez-Sebastián S, Bárcena J, Nuñez MC, Martínez-Alonso D, Dudognon B, Guijarro E, Escribano JM (2015) Improved production efficiency of virus-like particles by the baculovirus expression vector system. PLoS One 10(10):e0140039. https://doi.org/10.1371/journal.pone.0140039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. van Oers MM (2011) Opportunities and challenges for the baculovirus expression system. J Invertebr Pathol 107:S3–S15. https://doi.org/10.1016/j.jip.2011.05.001

    Article  CAS  PubMed  Google Scholar 

  7. van Oers MM, Pijlman GP, Vlak JM (2015) Thirty years of baculovirus–insect cell protein expression: from dark horse to mainstream technology. J Gen Virol 96(1):6–23. https://doi.org/10.1099/vir.0.067108-0

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. UQ Protein Expression Facility, The University of Queensland, Brisbane, Queensland, Australia

    Adetayo A. Adeniyi & Linda Hwee-Lin Lua

Authors
  1. Adetayo A. Adeniyi
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  2. Linda Hwee-Lin Lua
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Corresponding author

Correspondence to Linda Hwee-Lin Lua .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Adeniyi, A.A., Lua, L.HL. (2020). Protein Expression in the Baculovirus-Insect Cell Expression System. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

  • eBook Packages: Springer Protocols

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