Abstract
Pentose sugar present in ribonucleic acid reacts with hot acid to form furfural, which on reaction with orcinol and ferric chloride produces green color. Purine nucleotides are more reactive than pyrimidine. The green color can be quantitated using UV spectrophotometer or colorimeter at 665 nm.
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Jain, A., Jain, R., Jain, S. (2020). Estimation of RNA Using Orcinol Method. In: Basic Techniques in Biochemistry, Microbiology and Molecular Biology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9861-6_23
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DOI: https://doi.org/10.1007/978-1-4939-9861-6_23
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