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Trinucleotide Repeats pp 103–112Cite as

Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2056))

Abstract

Instability of trinucleotide and inverted repeats is a causative factor in the development of a number of neurological diseases, hereditary syndromes, and cancer. To understand the mechanisms that lead to repeat-induced genome destabilization it is important to identify factors that affect repeat metabolism. Here we present an approach that utilizes systematic and unbiased genome-wide screen in yeast Saccharomyces cerevisiae aimed to find genes that govern GAA/TTC and inverted repeat instability. These screens allowed for the identification of more than 30 mutants with increased fragility of both repeat motifs.

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Acknowledgments

We thank Dr. N. Degtyareva for critical reading of the manuscript and Dr. Y. Zhang for carrying out the screens. This work was supported by NIH grant R01GM129119 to KSL.

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Authors and Affiliations

  1. Institute for Bioengineering and Bioscience, School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA

    Wenying Guo & Kirill S. Lobachev

Authors
  1. Wenying Guo
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  2. Kirill S. Lobachev
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Corresponding author

Correspondence to Kirill S. Lobachev .

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Editors and Affiliations

  1. Department Genomes & Genetics, Institut Pasteur, Paris, France

    Guy-Franck Richard

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Guo, W., Lobachev, K.S. (2020). Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae. In: Richard, GF. (eds) Trinucleotide Repeats. Methods in Molecular Biology, vol 2056. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9784-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9784-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9783-1

  • Online ISBN: 978-1-4939-9784-8

  • eBook Packages: Springer Protocols

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