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Multiphoton Microscopy pp 225–250Cite as

Studying a Light Sensor with Light: Multiphoton Imaging in the Retina

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Part of the book series: Neuromethods ((NM,volume 148))

Abstract

Two-photon imaging of light stimulus-evoked neuronal activity has been used to study all neuron classes in the vertebrate retina, from the photoreceptors to the retinal ganglion cells. Clearly, the ability to study retinal circuits down to the level of single synapses or zoomed out at the level of complete populations of neurons has been a major asset in our understanding of this beautiful circuit. In this chapter, we discuss the possibilities and pitfalls of using an all-optical approach in this highly light-sensitive part of the brain.

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Authors and Affiliations

  1. Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany

    Thomas Euler, Katrin Franke & Tom Baden

  2. Werner Reichardt Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany

    Thomas Euler

  3. Bernstein Centre for Computational Neuroscience Tübingen, University of Tübingen, Tübingen, Germany

    Katrin Franke

  4. School of Life Sciences, University of Sussex, Brighton, UK

    Tom Baden

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  1. Thomas Euler
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  2. Katrin Franke
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  3. Tom Baden
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Correspondence to Thomas Euler .

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Editors and Affiliations

  1. Department of Biomedicine, University of Bergen, Bergen, Norway

    Espen Hartveit

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Euler, T., Franke, K., Baden, T. (2019). Studying a Light Sensor with Light: Multiphoton Imaging in the Retina. In: Hartveit, E. (eds) Multiphoton Microscopy. Neuromethods, vol 148. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9702-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9702-2_10

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